PMID- 27158602
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160509
LR  - 20201001
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 9
IP  - 3
DP  - 2016
TI  - Expression and effect of proline hydroxylase domain 2 in retina of diabetic rats.
PG  - 357-62
LID - 10.18240/ijo.2016.03.05 [doi]
AB  - AIM: To observe the expression of proline hydroxylase domain 2 (PHD2) in the 
      retina of diabetic rats and investigate the relationship between PHD2 and 
      relevant intraocular vascular proliferation factors. METHODS: Sixty male specific 
      pathogen free (SPF) Sprague-Dawley (SD) rats were randomly divided into two 
      groups: the diabetic group and the control group. The rats in the diabetic group 
      were intraperitoneally injected with 60 mg/kg (0.60 mL/100g) of streptozotocin to 
      induce a diabetic rat model. The rats in the control group were injected with an 
      equal volume of sodium citrate buffer solution by the same method. 
      Hematoxylin-eosin (HE) staining and immumofluorescence (IF) method were adopted 
      to observe the pathological changes of retinal tissues and the expression of 
      PHD2, glial fibrillary acidic protein (GFAP), vascular endothelial growth factor 
      (VEGF) by 8wk. RT-PCR method was applied to detect the expressions of mRNA of 
      PHD2, VEGF and GFAP. The relationship between PHD2 and other vascular 
      proliferation factors was analyzed. RESULTS: HE staining showed that there was 
      the retinal tissue edema in the diabetic group, and the arrangement was in 
      disorder, and proliferation could be seen. IF staining: in the retina of normal 
      rats, PHD2 was not expressed, GFAP and VEGF were mainly expressed in astrocytes; 
      while in the diabetic rats, PHD2, GFAP and VEGF staining showed strong positivity 
      in all retinal layers, mainly in neurogliocytes. PHD2 was co-expressed with VEGF 
      and GFAP. The mRNA expression levels of PHD2, GFAP and VEGF in the diabetic group 
      were obviously higher than that in the control group,respectively 1.83 times, 
      1.75 times and 2.08 times. The difference had statistical significance (P<0.01). 
      CONCLUSION: The high expression of PHD2 in the retina of early-stage diabetic 
      rats might result from secretion of neurogliocytes induced by local 
      high-concentration blood glucose, thus promoting the expression of VEGF and GFAP. 
      PHD2 plays an important role during the occurrence of diabetic retinopathy.
FAU - Li, Zhen
AU  - Li Z
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, 
      Hubei Province, China.
FAU - Xing, Yi-Qiao
AU  - Xing YQ
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, 
      Hubei Province, China.
FAU - Cui, Wei
AU  - Cui W
AD  - Department of Ophthalmology, Inner Mongolia People's Hospital, Hohhot 010017, 
      Inner Mongolia Autonomous Region, China.
FAU - Lu, Qiang
AU  - Lu Q
AD  - Department of Ophthalmology, Inner Mongolia People's Hospital, Hohhot 010017, 
      Inner Mongolia Autonomous Region, China.
LA  - eng
PT  - Journal Article
DEP - 20160318
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC4844037
OTO - NOTNLM
OT  - diabetic retinopathy
OT  - proline hydroxylase domain 2
OT  - retinal neovascularization
EDAT- 2016/05/10 06:00
MHDA- 2016/05/10 06:01
PMCR- 2016/03/18
CRDT- 2016/05/10 06:00
PHST- 2015/12/24 00:00 [received]
PHST- 2016/02/14 00:00 [accepted]
PHST- 2016/05/10 06:00 [entrez]
PHST- 2016/05/10 06:00 [pubmed]
PHST- 2016/05/10 06:01 [medline]
PHST- 2016/03/18 00:00 [pmc-release]
AID - ijo-09-03-357 [pii]
AID - 10.18240/ijo.2016.03.05 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2016 Mar 18;9(3):357-62. doi: 10.18240/ijo.2016.03.05. 
      eCollection 2016.