PMID- 27158894
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 5
DP  - 2016
TI  - Biphasic and Stage-Associated Expression of CPEB4 in Hepatocellular Carcinoma.
PG  - e0155025
LID - 10.1371/journal.pone.0155025 [doi]
LID - e0155025
AB  - Cytoplasmic polyadenylation element binding protein 4 (CPEB4) is a 
      sequence-specific RNA-binding protein and translational regulator, with 
      expression associated with tumor progression. Nevertheless, CPEB4 seems to play 
      paradoxical roles in cancers-an oncogenic promoter in pancreatic ductal 
      adenocarcinoma (PDA) and glioblastomas but a tumor suppressor in hepatocellular 
      carcinoma (HCC). To assess whether CPEB4-regulated carcinogenesis is 
      tissue-specific, we reevaluated the role of CPEB4 in HCC. Although proliferation 
      of hepatocytes appeared normal in CPEB4-knockout (KO) mice after partial 
      hepatectomy, knockdown (KD) of CPEB4 in HepG2 liver cancer cells promoted colony 
      formation in vitro. Moreover, the growth of CPEB4-KD cells was accelerated in an 
      in vivo xenograft mouse model. In tumorous and adjacent non-tumorous paired liver 
      specimens from 49 HCC patients, the protein level of CPEB4 was significantly 
      upregulated in early-stage HCC but decreased toward late-stage HCC. This finding 
      agrees with changes in CPEB4 mRNA level from analysis of two sets of HCC 
      microarray data from the Gene Expression Omnibus (GEO) database. Taken together, 
      downregulation of CPEB4 likely occurs at the late cancer stage to facilitate HCC 
      progression. Biphasic alteration of CPEB4 expression during HCC progression 
      suggests its complicated role in tumorigenesis.
FAU - Tsai, Li-Yun
AU  - Tsai LY
AD  - Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, 
      Taiwan.
FAU - Chang, Yu-Wei
AU  - Chang YW
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
FAU - Lee, Ming-Che
AU  - Lee MC
AD  - Department of Surgery, Buddhist Tzu Chi General Hospital and Tzu Chi University, 
      Hualien, Taiwan.
FAU - Chang, Ying-Chen
AU  - Chang YC
AD  - Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, 
      Taiwan.
FAU - Hwang, Pei-Ing
AU  - Hwang PI
AD  - General Manager, Mao Ying Genetech Inc., Taipei, Taiwan.
FAU - Huang, Yi-Shuian
AU  - Huang YS
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
FAU - Cheng, Ching-Feng
AU  - Cheng CF
AD  - Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, 
      Taiwan.
AD  - Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
AD  - Department of Pediatrics, Buddhist Tzu Chi General Hospital and Tzu Chi 
      University, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160509
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (CPEB4 protein, human)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Carcinoma, Hepatocellular/*metabolism
MH  - Female
MH  - Heterografts
MH  - Humans
MH  - Liver Neoplasms/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Middle Aged
MH  - RNA-Binding Proteins/genetics/immunology/*metabolism
PMC - PMC4861299
COIS- Competing Interests: PeiIng Hwang is employed by Mao Ying Genetech Inc. There are 
      no patents, products in development or marketed products to declare. This does 
      not alter the authors' adherence to all the PLOS ONE policies on sharing data and 
      materials, as detailed online in the guide for authors.
EDAT- 2016/05/10 06:00
MHDA- 2017/07/27 06:00
PMCR- 2016/05/09
CRDT- 2016/05/10 06:00
PHST- 2015/11/01 00:00 [received]
PHST- 2016/04/22 00:00 [accepted]
PHST- 2016/05/10 06:00 [entrez]
PHST- 2016/05/10 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
PHST- 2016/05/09 00:00 [pmc-release]
AID - PONE-D-15-47842 [pii]
AID - 10.1371/journal.pone.0155025 [doi]
PST - epublish
SO  - PLoS One. 2016 May 9;11(5):e0155025. doi: 10.1371/journal.pone.0155025. 
      eCollection 2016.