PMID- 27159259
OWN - NLM
STAT- MEDLINE
DCOM- 20170724
LR  - 20231213
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 45
IP  - 10
DP  - 2016 Nov
TI  - Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic 
      cheilitis and lip squamous cell carcinomas.
PG  - 774-779
LID - 10.1111/jop.12453 [doi]
AB  - BACKGROUND: Epigenetic modifications, including DNA methylation of tumor 
      suppressor genes carried out by DNA methyltransferases (DNMTs), are important 
      events in carcinogenesis. Although there are studies concerning to its expression 
      in several cancer types, DNMTs expression pattern is not known in photoinduced 
      lip carcinogenesis. The aim of this study was to investigate the immunoexpression 
      of DNMTs 1, 3a, and 3b in lip precancerous lesion (actinic cheilitis) and cancer. 
      METHODS: Thirty cases of actinic cheilitis (AC), thirty cases of lip squamous 
      cell carcinoma (LSCC), and twenty cases of non-neoplastic tissue (NNT) were 
      selected for immunohistochemical investigation of DNMTs 1, 3a, and 3b. RESULTS: 
      Nuclear DNMT 1 immunoreactivity was significantly higher in the LSCC group 
      (68.6%) compared with NNT (47%), and nuclear DNMT 3b was higher in LSCC (70.9%) 
      than in NNT (37.9%) and in AC (44%). Only DNMT 3a showed both higher nuclear and 
      cytoplasmic expression in AC (35.9% and 35.5%, respectively) than in NNT (4.4% 
      and 16.1%, respectively) and LSCC (8.8% and 13.2%, respectively) (P < 0.05). 
      CONCLUSIONS: The results suggested that DNMT 3a could play a key role in the 
      methylation process of initial steps of UV carcinogenesis present in AC while 
      DNMT 3b could be responsible for de novo methylation in already established lip 
      cancer.
CI  - (c) 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Daniel, Filipe I
AU  - Daniel FI
AD  - Pathology Department and Dentistry Graduate Program, Federal University of Santa 
      Catarina, Florianopolis, Santa Catarina, Brazil. filipe.daniel@ufsc.br.
FAU - Alves, Soraia R
AU  - Alves SR
AD  - Private Dentist, Florianopolis, Santa Catarina, Brazil.
FAU - Vieira, Daniella S C
AU  - Vieira DS
AD  - Pathology Department, Federal University of Santa Catarina, Florianopolis, Santa 
      Catarina, Brazil.
FAU - Biz, Michelle T
AU  - Biz MT
AD  - Morphology Sciences Department and Dentistry Graduate Program, Federal University 
      of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
FAU - Daniel, Inah W B S
AU  - Daniel IW
AD  - Pediatrics Department, Federal University of Santa Catarina, Florianopolis, Santa 
      Catarina, Brazil.
FAU - Modolo, Filipe
AU  - Modolo F
AUID- ORCID: 0000-0002-5395-0486
AD  - Pathology Department and Dentistry Graduate Program, Federal University of Santa 
      Catarina, Florianopolis, Santa Catarina, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20160509
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (DMAP1 protein, human)
RN  - 0 (DNMT3A protein, human)
RN  - 0 (Repressor Proteins)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA Methyltransferase 3A)
RN  - Actinic cheilitis
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinogenesis/immunology/metabolism/pathology
MH  - Carcinoma, Squamous Cell/*enzymology/immunology/pathology
MH  - Cheilitis/*enzymology/immunology/pathology
MH  - Child
MH  - Child, Preschool
MH  - DNA (Cytosine-5-)-Methyltransferases/*biosynthesis/metabolism
MH  - DNA Methyltransferase 3A
MH  - Female
MH  - Head and Neck Neoplasms/*enzymology/immunology/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lip Neoplasms/*enzymology/immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Repressor Proteins/*biosynthesis/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Young Adult
MH  - DNA Methyltransferase 3B
OTO - NOTNLM
OT  - DNA methylation
OT  - actinic cheilitis
OT  - lip cancer
OT  - methyltransferases
OT  - squamous cell carcinoma
EDAT- 2016/10/27 06:00
MHDA- 2017/07/25 06:00
CRDT- 2016/05/10 06:00
PHST- 2016/04/09 00:00 [accepted]
PHST- 2016/10/27 06:00 [pubmed]
PHST- 2017/07/25 06:00 [medline]
PHST- 2016/05/10 06:00 [entrez]
AID - 10.1111/jop.12453 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2016 Nov;45(10):774-779. doi: 10.1111/jop.12453. Epub 2016 May 
      9.