PMID- 27160416
OWN - NLM
STAT- MEDLINE
DCOM- 20180919
LR  - 20181004
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 141
IP  - 13
DP  - 2016 Jun 20
TI  - Correlating microscopy techniques and ToF-SIMS analysis of fully grown mammalian 
      oocytes.
PG  - 4121-9
LID - 10.1039/c6an00665e [doi]
AB  - The 2D-molecular thin film analysis protocol for fully grown mice oocytes is 
      described using an innovative approach. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force 
      microscopy (AFM) and optical microscopy imaging were applied to the same mice 
      oocyte section on the same sample holder. A freeze-dried mice oocyte was 
      infiltrated into embedding media, e.g. Epon, and then was cut with a microtome 
      and 2 mum thick sections were transferred onto an ITO coated conductive glass. 
      Mammalian oocytes can contain "nucleolus-like body" (NLB) units and ToF-SIMS 
      analysis was used to investigate the NLB composition. The ion-spatial 
      distribution in the cell components was identified and compared with the images 
      acquired by SEM, AFM and optical microscopy. This study presents a significant 
      advancement in cell embryology, cell physiology and cancer-cell biochemistry.
FAU - Gulin, Alexander
AU  - Gulin A
AD  - N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. 
      Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com and Moscow State 
      University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russian 
      Federation.
FAU - Nadtochenko, Victor
AU  - Nadtochenko V
AD  - N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. 
      Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com and Moscow State 
      University, Department of Chemistry, Leninskiye Gory 1-3, 119991, Moscow, Russian 
      Federation and Institute of Problems of Chemical Physics, Russian Academy of 
      Sciences, pr. Akademika Semenova 1, 142432 Chernogolovka, Moscow Region, Russia.
FAU - Astafiev, Artyom
AU  - Astafiev A
AD  - N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. 
      Kosygina 4, 119991 Moscow, Russia. nadtochenko@gmail.com.
FAU - Pogorelova, Valentina
AU  - Pogorelova V
AD  - Institute of Theoretical and Experimental Biophysics, RAS, ul. Institustskaya 3, 
      142290 Pushchino, Moscow Region, Russia.
FAU - Rtimi, Sami
AU  - Rtimi S
AD  - Ecole Polytechnique Fedeerale de Lausanne, Institute of chemical sciences and 
      engineering (ISIC), Lausanne, VD, CH 1015, Switzerland.
FAU - Pogorelov, Alexander
AU  - Pogorelov A
AD  - Institute of Theoretical and Experimental Biophysics, RAS, ul. Institustskaya 3, 
      142290 Pushchino, Moscow Region, Russia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
SB  - IM
MH  - Animals
MH  - Freeze Drying
MH  - Mice
MH  - *Microscopy, Atomic Force
MH  - *Microscopy, Electron, Scanning
MH  - Oocytes/*cytology
MH  - *Spectrometry, Mass, Secondary Ion
EDAT- 2016/05/11 06:00
MHDA- 2018/09/20 06:00
CRDT- 2016/05/11 06:00
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2018/09/20 06:00 [medline]
AID - 10.1039/c6an00665e [doi]
PST - ppublish
SO  - Analyst. 2016 Jun 20;141(13):4121-9. doi: 10.1039/c6an00665e.