PMID- 27160880
OWN - NLM
STAT- MEDLINE
DCOM- 20171213
LR  - 20181202
IS  - 1879-0097 (Electronic)
IS  - 0109-5641 (Linking)
VI  - 32
IP  - 7
DP  - 2016 Jul
TI  - Modulation of the nanometer pore size improves magnesium adsorption into 
      mesoporous titania coatings and promotes bone morphogenic protein 4 expression in 
      adhering osteoblasts.
PG  - e148-58
LID - S0109-5641(16)30046-X [pii]
LID - 10.1016/j.dental.2016.04.002 [doi]
AB  - OBJECTIVE: Mesoporous (MP) titania films used as implant coatings have recently 
      been considered as release systems for controlled administration of magnesium to 
      enhance initial osteoblast proliferation in vitro. Tuning of the pore size in 
      such titania films is aimed at increasing the osteogenic potential through 
      effects on the total loading capacity and the release profile of magnesium. 
      METHODS: In this study, evaporation-induced self-assembly (EISA) was used with 
      different structure-directing agents to form three mesoporous films with average 
      pore sizes of 2nm (MP1), 6nm (MP2) and 7nm (MP3). Mg adsorption and release was 
      monitored using quartz crystal microbalance with dissipation (QCM-D). The film 
      surfaces were characterized with atomic force microscopy (AFM), scanning electron 
      microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The effect of 
      different Mg release on osteogenesis was investigated in human fetal osteoblasts 
      (hFOB) using pre-designed osteogenesis arrays and real-time polymerase chain 
      reaction (RT-PCR). RESULTS: Results showed a sustained release from all the films 
      investigated, with higher magnesium adsorption into MP1 and MP3 films. No 
      significant differences were observed in the surface nanotopography of the films, 
      either with or without the presence of magnesium. MP3 films (7nm pore size) had 
      the greatest effect on osteogenesis, up-regulating 15 bone-related genes after 1 
      week of hFOB growth and significantly promoting bone morphogenic protein (BMP4) 
      expression after 3 weeks of growth. SIGNIFICANCE: The findings indicate that the 
      increase in pore width on the nano scale significantly enhanced the bioactivity 
      of the mesoporous coating, thus accelerating osteogenesis without creating 
      differences in surface roughness.
CI  - Copyright (c) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All 
      rights reserved.
FAU - Cecchinato, Francesca
AU  - Cecchinato F
AD  - Department of Prosthodontics, Faculty of Odontology, Malmo University, Sweden. 
      Electronic address: francesca.cecchinato@mah.se.
FAU - Atefyekta, Saba
AU  - Atefyekta S
AD  - Department of Chemistry and Chemical Engineering, Applied Chemistry, Chalmers 
      University of Technology, Gothenburg, Sweden.
FAU - Wennerberg, Ann
AU  - Wennerberg A
AD  - Department of Prosthodontics, Faculty of Odontology, Malmo University, Sweden.
FAU - Andersson, Martin
AU  - Andersson M
AD  - Department of Chemistry and Chemical Engineering, Applied Chemistry, Chalmers 
      University of Technology, Gothenburg, Sweden.
FAU - Jimbo, Ryo
AU  - Jimbo R
AD  - Department of Prosthodontics, Faculty of Odontology, Malmo University, Sweden; 
      Department of Applied Prosthodontics, Graduate School of Biomedical Sciences, 
      Nagasaki University, Nagasaki, Japan.
FAU - Davies, Julia R
AU  - Davies JR
AD  - Department of Oral Biology, Faculty of Odontology, Malmo University, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20160506
PL  - England
TA  - Dent Mater
JT  - Dental materials : official publication of the Academy of Dental Materials
JID - 8508040
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
RN  - I38ZP9992A (Magnesium)
MH  - Adsorption
MH  - Bone Morphogenetic Protein 4/*metabolism
MH  - Humans
MH  - *Magnesium
MH  - *Osteoblasts
MH  - Surface Properties
MH  - *Titanium
OTO - NOTNLM
OT  - Magnesium release
OT  - Mesoporous titania films
OT  - Osteogenesis
OT  - Pore size
OT  - Titanium implant
EDAT- 2016/05/11 06:00
MHDA- 2017/12/14 06:00
CRDT- 2016/05/11 06:00
PHST- 2015/10/12 00:00 [received]
PHST- 2016/03/18 00:00 [revised]
PHST- 2016/04/22 00:00 [accepted]
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2017/12/14 06:00 [medline]
AID - S0109-5641(16)30046-X [pii]
AID - 10.1016/j.dental.2016.04.002 [doi]
PST - ppublish
SO  - Dent Mater. 2016 Jul;32(7):e148-58. doi: 10.1016/j.dental.2016.04.002. Epub 2016 
      May 6.