PMID- 27161005
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20231111
IS  - 1476-511X (Electronic)
IS  - 1476-511X (Linking)
VI  - 15
DP  - 2016 May 10
TI  - N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors 
      level in hfat-1 transgenic pigs.
PG  - 89
LID - 10.1186/s12944-016-0259-7 [doi]
LID - 89
AB  - BACKGROUND: The consumption of n-3 polyunsaturated fatty acids (PUFAs) is 
      important to human health, especially in cases of cardiovascular disease. 
      Although beneficial effects of n-3 PUFAs have been observed in a number of 
      studies, the mechanisms involved in these effects have yet to be discovered. 
      METHODS: We generated hfat-1 transgenic pigs with traditional somatic cell 
      nuclear transfer (SCNT) technology. The fatty acid composition in ear tissue of 
      pigs were detected with gas chromatography. The cholesterol, triglycerides (TAG) 
      and inflammation mediators in circulation were investigated. RESULTS: The hfat-1 
      transgenic pigs were developed which accumulate high levels of n-3 PUFAs than 
      wild-types pigs. Gas chromatography results demonstrated that the total n-3 PUFAs 
      in the ear tissues of the transgenic founders were 2-fold higher than the 
      wild-type pigs. A lipid analysis demonstrated that the levels of TAG in the 
      transgenic pigs were decreased significantly. The basal levels of the 
      inflammation mediators tumor necrosis factor-alpha (TNF-alpha), monocyte chemoattractant 
      protein-1 (MCP-1) and interleukin-6 (IL-6) in transgenic pigs were inhibited 
      markedly compared with the wild-type pigs. CONCLUSIONS: These results suggest 
      that n-3 PUFAs accumulation in vivo may have beneficial effects on vascular and 
      hfat-1 transgenic pigs may be a useful tool for investigating the involved 
      mechanisms.
FAU - Liu, Xingxing
AU  - Liu X
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Pang, Daxin
AU  - Pang D
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Yuan, Ting
AU  - Yuan T
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Li, Zhuang
AU  - Li Z
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Li, Zhanjun
AU  - Li Z
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Zhang, Mingjun
AU  - Zhang M
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Ren, Wenzhi
AU  - Ren W
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Ouyang, Hongsheng
AU  - Ouyang H
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China.
FAU - Tang, Xiaochun
AU  - Tang X
AD  - Jilin Provincial Model Animal Engineering Research Center, College of Animal 
      Sciences, Jilin University, Xi'an Road, 5333#, Jilin, 130062, China. 
      xiaochuntang@jlu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160510
PL  - England
TA  - Lipids Health Dis
JT  - Lipids in health and disease
JID - 101147696
RN  - 0 (Cadherins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cholesterol, HDL)
RN  - 0 (FAT1 protein, human)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Interleukin-6)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - *Animals, Genetically Modified
MH  - Cadherins/*genetics
MH  - Chemokine CCL2/genetics
MH  - Cholesterol/blood/genetics
MH  - Cholesterol, HDL/blood/genetics
MH  - Fatty Acids, Omega-3/pharmacokinetics/*pharmacology
MH  - Female
MH  - Humans
MH  - Inflammation/*diet therapy/genetics
MH  - Interleukin-6/genetics
MH  - Male
MH  - Sus scrofa
MH  - Triglycerides/*blood/genetics
MH  - Tumor Necrosis Factor-alpha/genetics
PMC - PMC4862157
OTO - NOTNLM
OT  - Inflammation
OT  - Transgenic pig- fat-1
OT  - Triglyceride
OT  - n-3 polyunsaturated fatty acids
EDAT- 2016/05/11 06:00
MHDA- 2017/06/27 06:00
PMCR- 2016/05/10
CRDT- 2016/05/11 06:00
PHST- 2016/02/06 00:00 [received]
PHST- 2016/05/03 00:00 [accepted]
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
PHST- 2016/05/10 00:00 [pmc-release]
AID - 10.1186/s12944-016-0259-7 [pii]
AID - 259 [pii]
AID - 10.1186/s12944-016-0259-7 [doi]
PST - epublish
SO  - Lipids Health Dis. 2016 May 10;15:89. doi: 10.1186/s12944-016-0259-7.