PMID- 27161295
OWN - NLM
STAT- MEDLINE
DCOM- 20180418
LR  - 20181202
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 266
DP  - 2016 Sep
TI  - Postmortem detection of antibiotic-specific immunoglobulin E in the case of 
      anaphylactic death.
PG  - 14-17
LID - S0379-0738(16)30180-3 [pii]
LID - 10.1016/j.forsciint.2016.04.025 [doi]
AB  - An allergic anaphylactic reaction is a potentially fatal cascade consisting of an 
      initial sensitization, antigen exposure, crosslinking of immunoglobulin E (IgE) 
      specific for the antigen, activation of mast cells, and release of bioactive 
      substances. Postmortem diagnosis of anaphylaxis is challenging because of the 
      limited availability of antemortem history and minimal macroscopic evidence at 
      autopsy. The elevated activity of a neutral proteinase, such as tryptase, can be 
      a surrogate marker for the activation and degranulation of mast cells. However, 
      it does not directly indicate the involvement of antigen-specific IgE, which has 
      an important role in IgE-mediated allergic anaphylaxis. In the present study, we 
      examined blood from a case of death following infusion of the antibiotic 
      ceftriaxone (CTRX), with a control case for comparison. The aim was to detect IgE 
      specific for CTRX. A pull-down assay with N-hydroxysuccinimide-activated 
      sepharose identified IgE specific for CTRX only in the serum obtained from the 
      case of CTRX exposure, and not in the control case. The specificity of IgE was 
      confirmed by adsorption to an excess of CTRX, which resulted in the signal for 
      IgE disappearing in the pull-down assay. This antigen-specific IgE is a key 
      molecule in the IgE-mediated allergic anaphylaxis and seldom investigated in 
      postmortem examinations. Its detection can provide support for the postmortem 
      diagnosis of allergic anaphylaxis, especially when combined with an antemortem 
      history of allergen exposure and elevated neutral proteinase levels in serum.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Takahashi, Motonori
AU  - Takahashi M
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan. Electronic 
      address: genring@med.kobe-u.ac.jp.
FAU - Kondo, Takeshi
AU  - Kondo T
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan.
FAU - Morichika, Mai
AU  - Morichika M
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan.
FAU - Kuse, Azumi
AU  - Kuse A
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan.
FAU - Nakagawa, Kanako
AU  - Nakagawa K
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan.
FAU - Ueno, Yasuhiro
AU  - Ueno Y
AD  - Division of Legal Medicine, Department of Community Medicine and Social Health 
      Science, Kobe University Graduate School of Medicine, Hyogo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160427
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Anti-Bacterial Agents)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Anaphylaxis/blood/*chemically induced/*diagnosis/immunology
MH  - Anti-Bacterial Agents/adverse effects/*immunology
MH  - Diagnosis
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Mast Cells/enzymology
OTO - NOTNLM
OT  - Amine coupling
OT  - Anaphylaxis
OT  - Ceftriaxone
OT  - Pull-down assay
EDAT- 2016/05/11 06:00
MHDA- 2018/04/19 06:00
CRDT- 2016/05/11 06:00
PHST- 2015/12/28 00:00 [received]
PHST- 2016/03/30 00:00 [revised]
PHST- 2016/04/19 00:00 [accepted]
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2018/04/19 06:00 [medline]
AID - S0379-0738(16)30180-3 [pii]
AID - 10.1016/j.forsciint.2016.04.025 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2016 Sep;266:14-17. doi: 10.1016/j.forsciint.2016.04.025. Epub 
      2016 Apr 27.