PMID- 27161864
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20180104
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 15
IP  - 6
DP  - 2016 Jun 3
TI  - Changes in Healthy Human IgG Fc-Glycosylation after Birth and during Early 
      Childhood.
PG  - 1853-61
LID - 10.1021/acs.jproteome.6b00038 [doi]
AB  - Glycosylation on the fragment crystallizable (Fc) region of immunoglobulin G 
      (IgG) has a large influence on the interaction of the antibody with Fc gamma 
      receptors (FcgammaRs). IgG consists of four subclasses that all have distinct 
      affinities for the different FcgammaRs. Knowledge about the Fc-glycosylation in 
      healthy human is valuable as reference for new biomarkers and in the design of 
      biopharmaceuticals that rely on IgG Fc-glycosylation. Previously, 
      subclass-specific characterization of IgG Fc-glycosylation was performed for 
      healthy adults, pregnant women, and newborns. For young healthy children, 
      however, the subclass-specific description of IgG Fc-glycosylation is still 
      lacking. Therefore, we performed the IgG subclass-specific analysis of the 
      Fc-glycosylation of 130 healthy humans between birth and 40 years of age, 
      including 22 samples derived from the umbilical cords of newborns. The analysis 
      was performed by a previously published matrix-assisted laser 
      desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS) 
      workflow, including a derivatization step for the linkage-specific stabilization 
      of sialic acids. The characterization revealed that when children start to 
      produce their own IgG they have a decreased galactosylation, sialylation, and 
      bisection and an increased fucosylation compared with newborns. During childhood, 
      the fucosylation and sialylation decrease, whereas bisection increases and 
      galactosylation stays constant.
FAU - de Haan, Noortje
AU  - de Haan N
AD  - Leiden University Medical Center , Center for Proteomics and Metabolomics, 2300 
      RC Leiden, The Netherlands.
FAU - Reiding, Karli R
AU  - Reiding KR
AD  - Leiden University Medical Center , Center for Proteomics and Metabolomics, 2300 
      RC Leiden, The Netherlands.
FAU - Driessen, Gertjan
AU  - Driessen G
AD  - Erasmus MC , Pediatrics, 3000 CA Rotterdam, The Netherlands.
FAU - van der Burg, Mirjam
AU  - van der Burg M
AD  - Erasmus MC , Immunology, 3000 CA Rotterdam, The Netherlands.
FAU - Wuhrer, Manfred
AU  - Wuhrer M
AD  - Leiden University Medical Center , Center for Proteomics and Metabolomics, 2300 
      RC Leiden, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160519
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 28RYY2IV3F (Fucose)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Child
MH  - Child, Preschool
MH  - Fucose/metabolism
MH  - Glucose/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Immunoglobulin Fc Fragments/*chemistry
MH  - Immunoglobulin G/*chemistry
MH  - Infant
MH  - Infant, Newborn
MH  - N-Acetylneuraminic Acid/metabolism
MH  - *Proteomics
MH  - Young Adult
OTO - NOTNLM
OT  - Fc-glycosylation
OT  - MALDI-TOF-MS
OT  - N-glycans
OT  - aging
OT  - glycopeptides
OT  - immunoglobulin G (IgG)
OT  - newborns
OT  - young children
EDAT- 2016/05/11 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/05/11 06:00
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - 10.1021/acs.jproteome.6b00038 [doi]
PST - ppublish
SO  - J Proteome Res. 2016 Jun 3;15(6):1853-61. doi: 10.1021/acs.jproteome.6b00038. 
      Epub 2016 May 19.