PMID- 27163639
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20181202
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 475
IP  - 1
DP  - 2016 Jun 17
TI  - 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell 
      line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated 
      apoptosis pathway.
PG  - 37-43
LID - S0006-291X(16)30708-2 [pii]
LID - 10.1016/j.bbrc.2016.04.151 [doi]
AB  - Cancer cells are mainly dependent on glycolysis to generate adenosine 
      triphosphate (ATP) and intermediates required for cell growth and proliferation. 
      Thus, inhibition of glycolysis might be of therapeutic value in antitumor 
      treatment. Our previously studies had found that both 3-bromopyruvate (BP) and 
      sodium citrate (SCT) can inhibit tumor growth and proliferation in vitro and 
      in vivo. However, the mechanism involved in the BP and SCT mediated antitumor 
      activity is not entirely clear. In this work, it is demonstrated that BP inhibits 
      the enzyme hexokinase (HK) activity and SCT suppresses the phosphofructokinase 
      (PFK) activity respectively, both the two agents decrease viability, ATP 
      generation and lactate content in the human gastric cancer cell line MGC-803. 
      These effects are directly correlated with blockage of glycolysis. Furthermore, 
      BP and SCT can induce the characteristic manifestations of mitochondria-regulated 
      apoptosis, such as down-regulation of anti-apoptosis proteins Bcl-2 and Survivin, 
      up-regulation of pro-apoptosis protein Bax, activation of caspase-3, as well as 
      leakage of cytochrome c (Cyt-c). In summary, our results provided evidences that 
      BP and SCT inhibit the MGC-803 cells growth and proliferation might be correlated 
      with inhibiting glycolysis and promoting mitochondria-regulated apoptosis.
CI  - Copyright (c) 2016 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Guo, Xingyu
AU  - Guo X
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China.
FAU - Zhang, Xiaodong
AU  - Zhang X
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China.
FAU - Wang, Tingan
AU  - Wang T
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China. 
      Electronic address: moonsonlife@yahoo.com.
FAU - Xian, Shulin
AU  - Xian S
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China.
FAU - Lu, Yunfei
AU  - Lu Y
AD  - Department of Gastrointestinal and Gland Surgery, The First Affiliated Hospital 
      of Guangxi Medical University, 6 ShuangYong Road, Nanning, Guangxi 530021, China. 
      Electronic address: doctorlife@126.com.
LA  - eng
PT  - Journal Article
DEP - 20160506
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Citrates)
RN  - 0 (Pyruvates)
RN  - 1Q73Q2JULR (Sodium Citrate)
RN  - 63JMV04GRK (bromopyruvate)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Citrates/*pharmacology
MH  - Gastric Mucosa/metabolism
MH  - Glycolysis/*drug effects
MH  - Humans
MH  - Mitochondria/drug effects/metabolism/pathology
MH  - Pyruvates/*pharmacology
MH  - Sodium Citrate
MH  - Stomach/*drug effects/pathology
MH  - Stomach Neoplasms/*drug therapy/metabolism/pathology
OTO - NOTNLM
OT  - 3-Bromopyruvate
OT  - Apoptosis
OT  - Energetic metabolism
OT  - Gastric carcinoma
OT  - Glycolysis
OT  - Sodium citrate
EDAT- 2016/05/11 06:00
MHDA- 2017/05/10 06:00
CRDT- 2016/05/11 06:00
PHST- 2016/04/16 00:00 [received]
PHST- 2016/04/27 00:00 [accepted]
PHST- 2016/05/11 06:00 [entrez]
PHST- 2016/05/11 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
AID - S0006-291X(16)30708-2 [pii]
AID - 10.1016/j.bbrc.2016.04.151 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Jun 17;475(1):37-43. doi: 
      10.1016/j.bbrc.2016.04.151. Epub 2016 May 6.