PMID- 27164997
OWN - NLM
STAT- MEDLINE
DCOM- 20170214
LR  - 20170214
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 54
DP  - 2016 Jul
TI  - Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and 
      apoptosis in hepatopancreas of pacific white shrimp (Litopenaeus vannamei).
PG  - 523-8
LID - S1050-4648(16)30314-X [pii]
LID - 10.1016/j.fsi.2016.05.009 [doi]
AB  - Ammonia is one of major environmental pollutants in the aquatic system that poses 
      a great threat to the survival of shrimp. In the present study, the mRNA 
      expression of endoplasmic reticulum (ER) stress marker and unfolded protein 
      response (UPR) related genes, as well as the change of redox enzyme and apoptosis 
      were investigated in hepatopancreas of the pacific white shrimp, Litopenaeus 
      vannamei after the exposure of 20 mg L(-1) total ammonia nitrogen (TAN). Compared 
      with the control group, the superoxide dismutase (SOD) activity in hepatopancreas 
      decreased significantly (p < 0.05) at 96 h, whereas the malonyldialdehyde (MDA) 
      concentration increased significantly (p < 0.05). The mRNA expression levels of 
      ER stress marker-immunoglobulin heavy chain binding protein (Bip) gene and key 
      UPR related genes including activating transcription factor 4 (ATF4) and the 
      spliced form of X box binding protein 1 (XBP1) increased significantly (p < 0.05) 
      in hepatopancreas at 96 h after exposure to ammonia. In addition, apoptosis was 
      observed obviously in the hepatopancreas of L. vannamei after exposure to ammonia 
      by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) assay. The results indicated that ammonia exposure could induce oxidative 
      stress, which further caused ER stress and apoptosis in hepatopancreas of L. 
      vannamei.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Liang, Zhongxiu
AU  - Liang Z
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao, 266071, China.
FAU - Liu, Rui
AU  - Liu R
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao, 266071, China.
FAU - Zhao, Depeng
AU  - Zhao D
AD  - Dalian Polytechnic University, Dalian, 116034, China.
FAU - Wang, Lingling
AU  - Wang L
AD  - Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry 
      of Agriculture, Dalian Ocean University, Dalian, 116023, China.
FAU - Sun, Mingzhe
AU  - Sun M
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao, 266071, China.
FAU - Wang, Mengqiang
AU  - Wang M
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao, 266071, China.
FAU - Song, Linsheng
AU  - Song L
AD  - Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry 
      of Agriculture, Dalian Ocean University, Dalian, 116023, China. Electronic 
      address: lshsong@ms.qdio.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20160507
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*toxicity
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Hepatopancreas/drug effects
MH  - Oxidative Stress/*drug effects
MH  - Penaeidae/*drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Ammonia exposure
OT  - Apoptosis
OT  - Endoplasmic reticulum stress
OT  - Litopenaeus vannamei
OT  - Oxidative stress
EDAT- 2016/05/12 06:00
MHDA- 2017/02/15 06:00
CRDT- 2016/05/12 06:00
PHST- 2016/03/22 00:00 [received]
PHST- 2016/05/06 00:00 [revised]
PHST- 2016/05/06 00:00 [accepted]
PHST- 2016/05/12 06:00 [entrez]
PHST- 2016/05/12 06:00 [pubmed]
PHST- 2017/02/15 06:00 [medline]
AID - S1050-4648(16)30314-X [pii]
AID - 10.1016/j.fsi.2016.05.009 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Jul;54:523-8. doi: 10.1016/j.fsi.2016.05.009. Epub 
      2016 May 7.