PMID- 27166774
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20220629
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 71
IP  - 4
DP  - 2016 Apr
TI  - Clonidine-stimulated growth hormone concentrations (cut-off values) measured by 
      immunochemiluminescent assay (ICMA) in children and adolescents with short 
      stature.
PG  - 226-31
LID - 10.6061/clinics/2016(04)09 [doi]
AB  - OBJECTIVES: To establish cut-off values for growth hormone concentrations using 
      clonidine as a secretagogue and an immunochemiluminescent assay as the method of 
      measurement and to analyze the response time as well as the influence of gender, 
      nutritional status and pubertal stage. METHODS: A total of 225 tests were 
      performed in 3 patient groups, categorized as group 1 (normal), group 2 
      (idiopathic short stature) and group 3 (growth hormone deficiency). Among the 199 
      disease-free individuals, 138 were prepubertal, and 61 were pubertal. Clonidine 
      (0.1 mg/m2) was orally administered, and the growth hormone level was measured by 
      immunochemiluminescent assay. The growth hormone peak and the difference between 
      the growth hormone peak and the baseline level were then analyzed. Statistical 
      analyses were performed using Student's t-test or the Mann-Whitney test and 
      Kruskal-Wallis test followed by Dunn's post hoc test. Cut-off values were 
      determined using a receiver operating characteristic curve. RESULTS: Group 1 and 
      group 2 had no difference in growth hormone peak, gender, body mass index 
      standard deviation score, or pubertal stage. Group 3 exhibited a significantly 
      lower growth hormone peak than the other groups did. The receiver operating 
      characteristic curve demonstrated that growth hormone concentrations >/= 3.0 ng/mL 
      defined responsiveness to clonidine. In total, 3.02% of individuals in group 1 
      and group 2 were considered false positive, i.e., these children lacked growth 
      hormone deficiency and had a peak below 3.0 ng/mL. CONCLUSION: 
      Clonidine-stimulated growth hormone concentrations >/=3 ng/mL, as measured by 
      immunochemiluminescent assay, suggest responsiveness to the stimulus regardless 
      of gender, body mass index standard deviation score or pubertal stage.
FAU - Borges, Maria de Fatima
AU  - Borges Mde F
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Teixeira, Flavia Carolina Candida
AU  - Teixeira FC
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Feltrin, Aline Karin
AU  - Feltrin AK
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Ribeiro, Karina Alvarenga
AU  - Ribeiro KA
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Nascentes, Gabriel Antonio Nogueira
AU  - Nascentes GA
AD  - Microbiologia e Imunologia, Instituto Federal do Triangulo Mineiro, Uberaba, MG, 
      Brazil.
FAU - Resende, Elisabete Aparecida Mantovani Rodrigues
AU  - Resende EA
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Ferreira, Beatriz Pires
AU  - Ferreira BP
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Silva, Adriana Paula
AU  - Silva AP
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
FAU - Palhares, Heloisa Marcelina Cunha
AU  - Palhares HM
AD  - Unidade de Endocrinologia Pediatrica, Divisao de Endocrinologia, Universidade 
      Federal do Triangulo Mineiro, Uberaba, MG, Brazil.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - 0 (Adrenergic alpha-2 Receptor Agonists)
RN  - 12629-01-5 (Human Growth Hormone)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
RN  - MN3L5RMN02 (Clonidine)
SB  - IM
MH  - Adolescent
MH  - Adrenergic alpha-2 Receptor Agonists/*pharmacology
MH  - *Body Height
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Clonidine/*pharmacology
MH  - Female
MH  - Growth Disorders/blood/*diagnosis/etiology
MH  - Growth Hormone/blood/*deficiency
MH  - Human Growth Hormone/*blood
MH  - Humans
MH  - Immunoassay/methods
MH  - Insulin-Like Growth Factor I/analysis
MH  - Luminescent Measurements/methods
MH  - Male
MH  - Prospective Studies
MH  - ROC Curve
PMC - PMC4825198
COIS- No potential conflict of interest was reported.
EDAT- 2016/05/12 06:00
MHDA- 2017/04/05 06:00
PMCR- 2016/04/01
CRDT- 2016/05/12 06:00
PHST- 2015/10/29 00:00 [received]
PHST- 2016/02/04 00:00 [accepted]
PHST- 2016/05/12 06:00 [entrez]
PHST- 2016/05/12 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - S1807-5932(22)01113-9 [pii]
AID - cln_71p226 [pii]
AID - 10.6061/clinics/2016(04)09 [doi]
PST - ppublish
SO  - Clinics (Sao Paulo). 2016 Apr;71(4):226-31. doi: 10.6061/clinics/2016(04)09.