PMID- 27166856
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 110
DP  - 2016 Apr 18
TI  - An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets.
PG  - e53824
LID - 10.3791/53824 [doi]
LID - 53824
AB  - Dendritic cells (DCs) are professional antigen-presenting cells primarily 
      responsible for acquiring, processing and presenting antigens on antigen 
      presenting molecules to initiate T-cell-mediated immunity. Dendritic cells can be 
      separated into several phenotypically and functionally heterogeneous subsets. 
      Three important subsets of splenic dendritic cells are plasmacytoid, CD8alpha(Pos) 
      and CD8alpha(Neg) cells. The plasmacytoid DCs are natural producers of type I 
      interferon and are important for anti-viral T cell immunity. The CD8alpha(Neg) DC 
      subset is specialized for MHC class II antigen presentation and is centrally 
      involved in priming CD4 T cells. The CD8alpha(Pos) DCs are primarily responsible for 
      cross-presentation of exogenous antigens and CD8 T cell priming. The CD8alpha(Pos) 
      DCs have been demonstrated to be most efficient at the presentation of glycolipid 
      antigens by CD1d molecules to a specialized T cell population known as invariant 
      natural killer T (iNKT) cells. Administration of Flt-3 ligand increases the 
      frequency of migration of dendritic cell progenitors from bone marrow, ultimately 
      resulting in expansion of dendritic cells in peripheral lymphoid organs in murine 
      models. We have adapted this model to purify large numbers of functional 
      dendritic cells for use in cell transfer experiments to compare in vivo 
      proficiency of different DC subsets.
FAU - Arora, Pooja
AU  - Arora P
AD  - Department of Microbiology and Immunology, Albert Einstein College of Medicine.
FAU - Porcelli, Steven A
AU  - Porcelli SA
AD  - Department of Microbiology and Immunology, Albert Einstein College of Medicine; 
      Department of Medicine (Rheumatology), Albert Einstein College of Medicine; 
      steven.porcelli@einstein.yu.edu.
LA  - eng
GR  - R01 AI045889/AI/NIAID NIH HHS/United States
GR  - P30 AI051519/AI/NIAID NIH HHS/United States
GR  - AI45889/AI/NIAID NIH HHS/United States
GR  - AI51519/AI/NIAID NIH HHS/United States
GR  - CA013330/CA/NCI NIH HHS/United States
GR  - P30 CA013330/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20160418
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Antigens, CD1d)
RN  - 0 (CD1d antigen, mouse)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/cytology
MH  - Antigens, CD1d/metabolism
MH  - CD8 Antigens/metabolism
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Separation/*methods
MH  - Dendritic Cells/*cytology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Immunity, Cellular
MH  - Lymphocyte Activation/immunology
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Natural Killer T-Cells/immunology
MH  - Spleen/immunology
PMC - PMC4941942
EDAT- 2016/05/12 06:00
MHDA- 2016/12/15 06:00
PMCR- 2018/04/18
CRDT- 2016/05/12 06:00
PHST- 2016/05/12 06:00 [entrez]
PHST- 2016/05/12 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2018/04/18 00:00 [pmc-release]
AID - 53824 [pii]
AID - 10.3791/53824 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Apr 18;(110):e53824. doi: 10.3791/53824.