PMID- 27167458
OWN - NLM
STAT- MEDLINE
DCOM- 20170810
LR  - 20240325
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 110
DP  - 2016 Apr 16
TI  - Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina 
      pasteurii.
LID - 10.3791/53253 [doi]
LID - 53253
AB  - The particular bacterium under investigation here (S. pasteurii) is unique in its 
      ability, under the right conditions, to induce the hydrolysis of urea (ureolysis) 
      in naturally occurring environments through secretion of an enzyme urease. This 
      process of ureolysis, through a chain of chemical reactions, leads to the 
      formation of calcium carbonate precipitates. This is known as Microbiologically 
      Induced Calcite Precipitation (MICP). The proper culture protocols for MICP are 
      detailed here. Finally, visualization experiments under different modes of 
      microscopy were performed to understand various aspects of the precipitation 
      process. Techniques like optical microscopy, Scanning Electron Microscopy (SEM) 
      and X-Ray Photo-electron Spectroscopy (XPS) were employed to chemically 
      characterize the end-product. Further, the ability of these precipitates to clog 
      pores inside a natural porous medium was demonstrated through a qualitative 
      experiment where sponge bars were used to mimic a pore-network with a range of 
      length scales. A sponge bar dipped in the culture medium containing the bacterial 
      cells hardens due to the clogging of its pores resulting from the continuous 
      process of chemical precipitation. This hardened sponge bar exhibits superior 
      strength when compared to a control sponge bar which becomes compressed and 
      squeezed under the action of an applied external load, while the hardened bar is 
      able to support the same weight with little deformation.
FAU - Bhaduri, Swayamdipta
AU  - Bhaduri S
AD  - Department of Mechanical Engineering, University of Alberta.
FAU - Debnath, Nandini
AU  - Debnath N
AD  - Department of Mechanical Engineering, University of Alberta.
FAU - Mitra, Sushanta
AU  - Mitra S
AD  - Department of Mechanical Engineering, York University.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Civil and Environmental Engineering, University of Alberta.
FAU - Kumar, Aloke
AU  - Kumar A
AD  - Department of Mechanical Engineering, University of Alberta; 
      aloke.kumar@ualberta.ca.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20160416
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 8W8T17847W (Urea)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Calcium Carbonate/analysis/*chemistry
MH  - *Chemical Precipitation
MH  - Sporosarcina/*enzymology
MH  - Urea
MH  - Urease/*biosynthesis
PMC - PMC4941918
EDAT- 2016/05/12 06:00
MHDA- 2017/08/11 06:00
PMCR- 2018/04/16
CRDT- 2016/05/12 06:00
PHST- 2016/05/12 06:00 [entrez]
PHST- 2016/05/12 06:00 [pubmed]
PHST- 2017/08/11 06:00 [medline]
PHST- 2018/04/16 00:00 [pmc-release]
AID - 53253 [pii]
AID - 10.3791/53253 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Apr 16;(110):53253. doi: 10.3791/53253.