PMID- 27167492
OWN - NLM
STAT- MEDLINE
DCOM- 20170707
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 110
DP  - 2016 Apr 27
TI  - In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart.
LID - 10.3791/53993 [doi]
LID - 53993
AB  - During embryogenesis, the epicardial contribution to coronary vasculature 
      development has been very well established. Cells derived from the epicardium 
      differentiate into smooth muscle cells, fibroblasts and endothelial cells that 
      contribute to the formation of coronary vessels. Here we have established an in 
      vitro culture method for embryonic epicardial cells. Using genetic labelling, we 
      have demonstrated that the majority of the migrating cells in our explant culture 
      are of epicardial origin. Epicardial explant cells also retain the expression of 
      epicardial markers (Wt1 and Tbx18). Furthermore, we provide evidence that 
      epicardial explant cells undergo epithelial to mesenchymal transition (EMT), 
      migrate and differentiate into smooth muscle cells after Transforming growth 
      factor beta 1 (TGF-beta1) treatment in a manner indistinguishable from that of 
      epicardial cells in vivo. In conclusion, we provide a novel method for the 
      culture of embryonic epicardial cells, which will help to explore the role of 
      specific genes in epicardial cell biology.
FAU - Ramesh, Sindhu
AU  - Ramesh S
AD  - Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical 
      School.
FAU - Singh, Anamika
AU  - Singh A
AD  - Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical 
      School.
FAU - Cibi, Dasan M
AU  - Cibi DM
AD  - Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical 
      School.
FAU - Hausenloy, Derek J
AU  - Hausenloy DJ
AD  - Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical 
      School; National Heart Research Institute Singapore, National Heart Centre 
      Singapore; The Hatter Cardiovascular Insititute, University College London.
FAU - Singh, Manvendra K
AU  - Singh MK
AD  - Program in Cardiovascular and Metabolic Disorders, Duke-NUS Graduate Medical 
      School; National Heart Research Institute Singapore, National Heart Centre 
      Singapore; manvendra.singh@duke-nus.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20160427
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/physiology
MH  - Cell Movement/physiology
MH  - Coronary Vessels/cytology/embryology
MH  - Epithelial-Mesenchymal Transition
MH  - Female
MH  - Fibroblasts/cytology
MH  - Mice
MH  - Multipotent Stem Cells/cytology
MH  - Myocytes, Smooth Muscle/cytology
MH  - Organogenesis
MH  - Pericardium/*cytology/embryology
MH  - Pregnancy
PMC - PMC4942012
EDAT- 2016/05/12 06:00
MHDA- 2017/07/08 06:00
PMCR- 2018/04/27
CRDT- 2016/05/12 06:00
PHST- 2016/05/12 06:00 [entrez]
PHST- 2016/05/12 06:00 [pubmed]
PHST- 2017/07/08 06:00 [medline]
PHST- 2018/04/27 00:00 [pmc-release]
AID - 53993 [pii]
AID - 10.3791/53993 [doi]
PST - epublish
SO  - J Vis Exp. 2016 Apr 27;(110):53993. doi: 10.3791/53993.