PMID- 27170009
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20191209
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 97
IP  - 8
DP  - 2016 Aug
TI  - The putative U94 integrase is dispensable for human herpesvirus 6 (HHV-6) 
      chromosomal integration.
PG  - 1899-1903
LID - 10.1099/jgv.0.000502 [doi]
AB  - Human herpesvirus 6 (HHV-6) can integrate its genome into the telomeres of host 
      chromosomes and is present in the germline of about 1 % of the human population. 
      HHV-6 encodes a putative integrase U94 that possesses all molecular functions 
      required for recombination including DNA-binding, ATPase, helicase and nuclease 
      activity, and was hypothesized by many researchers to facilitate integration ever 
      since the discovery of HHV-6 integration. However, analysis of U94 in the virus 
      context has been hampered by the lack of reverse-genetic systems and efficient 
      integration assays. Here, we addressed the role of U94 and the cellular 
      recombinase Rad51 in HHV-6 integration. Surprisingly, we could demonstrate that 
      HHV-6 efficiently integrated in the absence of U94 using a new quantitative 
      integration assay. Additional inhibition of the cellular recombinase Rad51 had 
      only a minor impact on virus integration. Our results shed light on this complex 
      integration mechanism that includes factors beyond U94 and Rad51.
FAU - Wallaschek, Nina
AU  - Wallaschek N
AD  - Institut fur Virologie, Freie Universitat Berlin, Robert von Ostertag-Strasse 
      7-13, 14163 Berlin, Germany.
FAU - Gravel, Annie
AU  - Gravel A
AD  - Division of Infectious Disease and Immunity, CHU de Quebec Research Center, 
      Quebec city, Quebec G1V 4G2, Canada.
FAU - Flamand, Louis
AU  - Flamand L
AD  - Division of Infectious Disease and Immunity, CHU de Quebec Research Center, 
      Quebec city, Quebec G1V 4G2, Canada.
AD  - Department of Microbiology, Infectious Disease and Immunology, Faculty of 
      Medicine, Universite Laval, Quebec city, Quebec, G1V 0A6, Canada.
FAU - Kaufer, Benedikt B
AU  - Kaufer BB
AD  - Institut fur Virologie, Freie Universitat Berlin, Robert von Ostertag-Strasse 
      7-13, 14163 Berlin, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160511
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 2.7.7.- (RAD51 protein, human)
RN  - EC 2.7.7.- (Rad51 Recombinase)
SB  - IM
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Herpesvirus 6, Human/genetics/*physiology
MH  - Humans
MH  - Integrases/genetics/*metabolism
MH  - Rad51 Recombinase/metabolism
MH  - Viral Proteins/genetics/*metabolism
MH  - *Virus Integration
EDAT- 2016/05/14 06:00
MHDA- 2017/05/10 06:00
CRDT- 2016/05/13 06:00
PHST- 2016/05/13 06:00 [entrez]
PHST- 2016/05/14 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
AID - 10.1099/jgv.0.000502 [doi]
PST - ppublish
SO  - J Gen Virol. 2016 Aug;97(8):1899-1903. doi: 10.1099/jgv.0.000502. Epub 2016 May 
      11.