PMID- 27173396
OWN - NLM
STAT- MEDLINE
DCOM- 20180124
LR  - 20181113
IS  - 1538-2443 (Electronic)
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 22
IP  - 5
DP  - 2016 Oct
TI  - Alphaherpesvirus DNA replication in dissociated human trigeminal ganglia.
PG  - 688-694
AB  - Analysis of the frequency and PCR-quantifiable abundance of herpes simplex virus 
      type 1 (HSV-1) and varicella zoster virus (VZV) DNA in multiple biological 
      replicates of cells from dissociated randomly distributed human trigeminal 
      ganglia (TG) of four subjects revealed an increase in both parameters and in both 
      viruses during 5 days of culture, with no further change by 10 days. Dissociated 
      TG provides a platform to analyze initiation of latent virus DNA replication 
      within 5 days of culture.
FAU - Cohrs, Randall J
AU  - Cohrs RJ
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA. randall.cohrs@ucdenver.edu.
AD  - Department of Immunology and Microbiology, University of Colorado School of 
      Medicine, Aurora, CO, 80045, USA. randall.cohrs@ucdenver.edu.
FAU - Badani, Hussain
AU  - Badani H
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
FAU - Bos, Nathan
AU  - Bos N
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
FAU - Scianna, Charles
AU  - Scianna C
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
FAU - Hoskins, Ian
AU  - Hoskins I
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
FAU - Baird, Nicholas L
AU  - Baird NL
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
FAU - Gilden, Don
AU  - Gilden D
AD  - Department of Neurology, University of Colorado School of Medicine, 12700 E. 19th 
      Avenue, Mail Stop B182, Aurora, CO, 80045, USA.
AD  - Department of Immunology and Microbiology, University of Colorado School of 
      Medicine, Aurora, CO, 80045, USA.
LA  - eng
GR  - P01 AG032958/AG/NIA NIH HHS/United States
GR  - R01 NS082228/NS/NINDS NIH HHS/United States
GR  - R01 NS093716/NS/NINDS NIH HHS/United States
GR  - T32 NS007321/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160512
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Autopsy
MH  - *DNA Replication
MH  - Female
MH  - Herpesvirus 1, Human/*physiology
MH  - Herpesvirus 3, Human/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Tissue Culture Techniques
MH  - Trigeminal Ganglion/*virology
MH  - *Virus Activation
MH  - Virus Latency
MH  - *Virus Replication
PMC - PMC5055419
MID - NIHMS792594
OTO - NOTNLM
OT  - DNA
OT  - HSV-1
OT  - Human trigeminal ganglia
OT  - Reactivation
OT  - VZV
COIS- All authors report no conflict of interest.
EDAT- 2016/05/14 06:00
MHDA- 2018/01/25 06:00
PMCR- 2017/10/01
CRDT- 2016/05/14 06:00
PHST- 2016/04/05 00:00 [received]
PHST- 2016/05/02 00:00 [accepted]
PHST- 2016/04/25 00:00 [revised]
PHST- 2016/05/14 06:00 [pubmed]
PHST- 2018/01/25 06:00 [medline]
PHST- 2016/05/14 06:00 [entrez]
PHST- 2017/10/01 00:00 [pmc-release]
AID - 10.1007/s13365-016-0450-7 [pii]
AID - 10.1007/s13365-016-0450-7 [doi]
PST - ppublish
SO  - J Neurovirol. 2016 Oct;22(5):688-694. doi: 10.1007/s13365-016-0450-7. Epub 2016 
      May 12.