PMID- 27173449 OWN - NLM STAT- MEDLINE DCOM- 20180108 LR - 20240325 IS - 1099-1492 (Electronic) IS - 0952-3480 (Print) IS - 0952-3480 (Linking) VI - 29 IP - 7 DP - 2016 Jul TI - Brain gamma-aminobutyric acid (GABA) detection in vivo with the J-editing (1) H MRS technique: a comprehensive methodological evaluation of sensitivity enhancement, macromolecule contamination and test-retest reliability. PG - 932-42 LID - 10.1002/nbm.3539 [doi] AB - Abnormalities in brain gamma-aminobutyric acid (GABA) have been implicated in various neuropsychiatric and neurological disorders. However, in vivo GABA detection by (1) H MRS presents significant challenges arising from the low brain concentration, overlap by much stronger resonances and contamination by mobile macromolecule (MM) signals. This study addresses these impediments to reliable brain GABA detection with the J-editing difference technique on a 3-T MR system in healthy human subjects by: (i) assessing the sensitivity gains attainable with an eight-channel phased-array head coil; (ii) determining the magnitude and anatomic variation of the contamination of GABA by MM; and (iii) estimating the test-retest reliability of the measurement of GABA with this method. Sensitivity gains and test-retest reliability were examined in the dorsolateral prefrontal cortex (DLPFC), whereas MM levels were compared across three cortical regions: DLPFC, the medial prefrontal cortex (MPFC) and the occipital cortex (OCC). A three-fold higher GABA detection sensitivity was attained with the eight-channel head coil compared with the standard single-channel head coil in DLPFC. Despite significant anatomical variation in GABA + MM and MM across the three brain regions (p < 0.05), the contribution of MM to GABA + MM was relatively stable across the three voxels, ranging from 41% to 49%, a non-significant regional variation (p = 0.58). The test-retest reliability of GABA measurement, expressed as either the ratio to voxel tissue water (W) or to total creatine, was found to be very high for both the single-channel coil and the eight-channel phased-array coil. For the eight-channel coil, for example, Pearson's correlation coefficient of test vs. retest for GABA/W was 0.98 (R(2) = 0.96, p = 0.0007), the percentage coefficient of variation (CV) was 1.25% and the intraclass correlation coefficient (ICC) was 0.98. Similar reliability was also found for the co-edited resonance of combined glutamate and glutamine (Glx) for both coils. Copyright (c) 2016 John Wiley & Sons, Ltd. CI - Copyright (c) 2016 John Wiley & Sons, Ltd. FAU - Shungu, Dikoma C AU - Shungu DC AD - Department of Radiology, Weill Cornell Medical College, New York, NY, USA. FAU - Mao, Xiangling AU - Mao X AD - Department of Radiology, Weill Cornell Medical College, New York, NY, USA. FAU - Gonzales, Robyn AU - Gonzales R AD - Department of Psychiatry, Columbia University, New York, NY, USA. FAU - Soones, Tacara N AU - Soones TN AD - Department of Psychiatry, Columbia University, New York, NY, USA. FAU - Dyke, Jonathan P AU - Dyke JP AD - Department of Radiology, Weill Cornell Medical College, New York, NY, USA. FAU - van der Veen, Jan Willem AU - van der Veen JW AD - NIMH Magnetic Resonance Spectroscopy Core Unit, Bethesda, MD, USA. FAU - Kegeles, Lawrence S AU - Kegeles LS AD - Department of Psychiatry, Columbia University, New York, NY, USA. AD - Department of Radiology, Columbia University, New York, NY, USA. LA - eng GR - R01 MH075895/MH/NIMH NIH HHS/United States GR - S10 OD021782/OD/NIH HHS/United States PT - Evaluation Study PT - Journal Article PT - Validation Study DEP - 20160513 PL - England TA - NMR Biomed JT - NMR in biomedicine JID - 8915233 RN - 0 (Macromolecular Substances) RN - 56-12-2 (gamma-Aminobutyric Acid) SB - IM MH - Adult MH - *Algorithms MH - Female MH - Humans MH - Macromolecular Substances/analysis/chemistry MH - Magnetic Resonance Imaging/*methods MH - Male MH - Molecular Imaging/methods MH - Prefrontal Cortex/*chemistry MH - Proton Magnetic Resonance Spectroscopy/*methods MH - Reproducibility of Results MH - Sensitivity and Specificity MH - *Signal Processing, Computer-Assisted MH - gamma-Aminobutyric Acid/*analysis PMC - PMC4909570 MID - NIHMS787727 OTO - NOTNLM OT - 1H MRS OT - GABA OT - Glutamate OT - Glx OT - J-editing OT - mobile macromolecules OT - phased-array coil OT - test-retest reliability EDAT- 2016/05/14 06:00 MHDA- 2018/01/09 06:00 PMCR- 2017/07/01 CRDT- 2016/05/14 06:00 PHST- 2015/09/09 00:00 [received] PHST- 2016/03/17 00:00 [revised] PHST- 2016/03/21 00:00 [accepted] PHST- 2016/05/14 06:00 [entrez] PHST- 2016/05/14 06:00 [pubmed] PHST- 2018/01/09 06:00 [medline] PHST- 2017/07/01 00:00 [pmc-release] AID - 10.1002/nbm.3539 [doi] PST - ppublish SO - NMR Biomed. 2016 Jul;29(7):932-42. doi: 10.1002/nbm.3539. Epub 2016 May 13.