PMID- 27180283
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20191210
IS  - 1876-7737 (Electronic)
IS  - 1874-3919 (Linking)
VI  - 142
DP  - 2016 Jun 16
TI  - Quantitative interactome reveals that porcine reproductive and respiratory 
      syndrome virus nonstructural protein 2 forms a complex with viral nucleocapsid 
      protein and cellular vimentin.
PG  - 70-81
LID - S1874-3919(16)30192-0 [pii]
LID - 10.1016/j.jprot.2016.05.009 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus 
      that has heavily impacted the global swine industry. The PRRSV nonstructural 
      protein 2 (nsp2) plays crucial roles in viral replication and host immune 
      regulation, most likely by interacting with viral or cellular proteins that have 
      not yet been identified. In this study, a quantitative interactome approach based 
      on immunoprecipitation and stable isotope labeling with amino acids in cell 
      culture (SILAC) was performed to identify nsp2-interacting proteins in 
      PRRSV-infected cells with an nsp2-specific monoclonal antibody. Nine viral 
      proteins and 62 cellular proteins were identified as potential nsp2-interacting 
      partners. Our data demonstrate that the PRRSV nsp1alpha, nsp1beta, and nucleocapsid 
      proteins all interact directly with nsp2. Nsp2-interacting cellular proteins were 
      classified into different functional groups and an interactome network of nsp2 
      was generated. Interestingly, cellular vimentin, a known receptor for PRRSV, 
      forms a complex with nsp2 by using viral nucleocapsid protein as an intermediate. 
      Taken together, the nsp2 interactome under the condition of virus infection 
      clarifies a role of nsp2 in PRRSV replication and immune evasion. BIOLOGICAL 
      SIGNIFICANCE: Viral proteins must interact with other virus-encoded proteins 
      and/or host cellular proteins to function, and interactome analysis is an ideal 
      approach for identifying such interacting proteins. In this study, we used the 
      quantitative interactome methodology to identify the viral and cellular proteins 
      that potentially interact with the nonstructural protein 2 (nsp2) of porcine 
      reproductive and respiratory syndrome virus (PRRSV) under virus infection 
      conditions, thus providing a rich source of potential viral and cellular 
      interaction partners for PRRSV nsp2. Based on the interactome data, we further 
      demonstrated that PRRSV nsp2 and nucleocapsid protein together with cellular 
      vimentin, form a complex that may be essential for viral attachment and 
      replication, which partly explains the role of nsp2 in PRRSV replication and 
      immune evasion.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Song, Tao
AU  - Song T
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China; The 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, PR 
      China.
FAU - Wang, Dang
AU  - Wang D
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - Zhang, Ruoxi
AU  - Zhang R
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - Zeng, Songlin
AU  - Zeng S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - An, Kang
AU  - An K
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China.
FAU - Chen, Huanchun
AU  - Chen H
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China; The 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, PR 
      China.
FAU - Xiao, Shaobo
AU  - Xiao S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan, Hubei, PR China; The 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, PR 
      China. Electronic address: vet@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160512
PL  - Netherlands
TA  - J Proteomics
JT  - Journal of proteomics
JID - 101475056
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Vimentin)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - HEK293 Cells
MH  - Humans
MH  - Nucleocapsid Proteins/*chemistry
MH  - Porcine respiratory and reproductive syndrome virus/*chemistry
MH  - *Protein Interaction Maps
MH  - *Protein Multimerization
MH  - Swine
MH  - Vimentin/*chemistry
MH  - Viral Nonstructural Proteins/*chemistry
MH  - Viral Proteins/chemistry
OTO - NOTNLM
OT  - Immunoprecipitation
OT  - Interactome
OT  - Nonstructural protein 2 (nsp2)
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Stable isotope labeling with amino acids in cell culture (SILAC)
EDAT- 2016/05/18 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/05/16 06:00
PHST- 2015/11/09 00:00 [received]
PHST- 2016/04/17 00:00 [revised]
PHST- 2016/05/07 00:00 [accepted]
PHST- 2016/05/16 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - S1874-3919(16)30192-0 [pii]
AID - 10.1016/j.jprot.2016.05.009 [doi]
PST - ppublish
SO  - J Proteomics. 2016 Jun 16;142:70-81. doi: 10.1016/j.jprot.2016.05.009. Epub 2016 
      May 12.