PMID- 27181873
OWN - NLM
STAT- MEDLINE
DCOM- 20170217
LR  - 20210109
IS  - 2040-1124 (Electronic)
IS  - 2040-1116 (Print)
IS  - 2040-1116 (Linking)
VI  - 7
IP  - 4
DP  - 2016 Jul
TI  - Comparison of the effect of bone marrow cells infusion through the portal vein 
      and inferior vena cava combined with short-term rapamycin on allogeneic islet 
      grafts in diabetic rats.
PG  - 476-84
LID - 10.1111/jdi.12456 [doi]
AB  - AIMS/INTRODUCTION: The study aimed to compare the impact of allogeneic bone 
      marrow cells (BMCs) infusion through the inferior vena cava (IVC) and portal vein 
      (PV) combined with rapamycin on allogeneic islet grafts in diabetic rats. 
      MATERIALS AND METHODS: Recipient diabetic Wistar rats were infused with islets 
      from Sprague-Dawley rats through the PV. PKH26-labeled BMCs of Sprague-Dawley 
      rats were infused to recipients through the PV or IVC, followed by administration 
      of rapamycin for 4 days. Blood glucose level was measured to evaluate the 
      survival time of the islets. Lymphocytes separated from blood, BMCs, thymus, 
      liver, spleen and lymph node were analyzed by flow cytometry. The peripheral 
      blood smear, BMCs smear and frozen sections of tissues were observed by a 
      fluorescence microscope. RESULTS: The survival time of the islets was 
      significantly prolonged by the BMCs infusion combined with rapamycin. The rats 
      receiving BMCs infusion through the PV induced a significantly longer survival 
      time of the islets, and increased mixed chimeras of allogeneic BMCs in the 
      thymus, liver, spleen and lymph node compared with the rats receiving BMCs 
      infusion through the IVC. The amount of the mixed chimeras on day 14 was lower 
      than that on day 7 after islet transplantation. Furthermore, PV transplantation 
      had significantly more mixed chimera than IVC transplantation in all analyzed 
      organs or tissues. CONCLUSIONS: BMCs infusion combined with rapamycin prolongs 
      the islets survival and induces mixed chimeras of BMCs. PV infusion of BMCs might 
      be a more effective strategy than IVC infusion of BMCs.
CI  - (c) 2015 The Authors. Journal of Diabetes Investigation published by Asian 
      Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, 
      Ltd.
FAU - Gao, Qingzhen
AU  - Gao Q
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Wang, Xiaoping
AU  - Wang X
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Zhang, Ruibin
AU  - Zhang R
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Wang, Pu
AU  - Wang P
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Jing, Yongsheng
AU  - Jing Y
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Ren, Wanjun
AU  - Ren W
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
FAU - Zhu, Bin
AU  - Zhu B
AD  - Department of Nephrology, Jinan Central Hospital Affiliated to Shandong 
      University, Jinan, Shandong Province, China.
LA  - eng
PT  - Journal Article
DEP - 20160123
PL  - Japan
TA  - J Diabetes Investig
JT  - Journal of diabetes investigation
JID - 101520702
RN  - 0 (Blood Glucose)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Bone Marrow Cells/drug effects/*physiology
MH  - Bone Marrow Transplantation/*methods
MH  - Diabetes Mellitus, Experimental/*surgery
MH  - Disease Models, Animal
MH  - Graft Survival
MH  - Islets of Langerhans Transplantation/*methods
MH  - Male
MH  - Portal Vein
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Sirolimus/*administration & dosage
MH  - Tissue Distribution
MH  - Transplantation, Homologous/methods
MH  - Vena Cava, Inferior
PMC - PMC4931197
OTO - NOTNLM
OT  - Bone marrow cell
OT  - Islet grafts
OT  - Rapamycin
EDAT- 2016/05/18 06:00
MHDA- 2017/02/18 06:00
PMCR- 2016/07/01
CRDT- 2016/05/17 06:00
PHST- 2015/06/30 00:00 [received]
PHST- 2015/11/23 00:00 [revised]
PHST- 2015/12/03 00:00 [accepted]
PHST- 2016/05/17 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/02/18 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - JDI12456 [pii]
AID - 10.1111/jdi.12456 [doi]
PST - ppublish
SO  - J Diabetes Investig. 2016 Jul;7(4):476-84. doi: 10.1111/jdi.12456. Epub 2016 Jan 
      23.