PMID- 27182983
OWN - NLM
STAT- MEDLINE
DCOM- 20170707
LR  - 20220311
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 5
DP  - 2016
TI  - Divergent Development of Hexaploid Triticale by a Wheat - Rye -Psathyrostachys 
      huashanica Trigeneric Hybrid Method.
PG  - e0155667
LID - 10.1371/journal.pone.0155667 [doi]
LID - e0155667
AB  - Hexaploid triticale is an important forage crop and a promising energy plant. 
      Some forms were previously reported for developing the hexaploid triticale, such 
      as crossing tetraploid wheat or hexaploid wheat with rye, crossing hexaploid 
      triticale and/or hexaploid wheat with octoploid triticale, and spontaneously 
      appearing in the selfed progenies of octoploid triticale. In the present study, 
      we developed an effective method for production of diverse types of hexaploid 
      triticale via wheat-rye-Psathyrostachys huashanica trigeneric hybrid. Genomic in 
      situ hybridization (GISH) and fluorescence in situ hybridization (FISH) 
      karyotyping revealed that D genome chromosomes were completely eliminated and the 
      whole A, B, and R genome chromosomes were retained in three lines. More 
      interestingly, the composite genome of the line K14-489-2 consisted of complete A 
      and B genomes and chromosomes 1D, 2R, 3R, 4R, 5R, 6R, and 7R, that of line 
      K14-491-2 was 12 A-genome (1A-6A), 14 B-genome (1B-7B), 12 R-genome (1R-3R, 
      5R-7R), and chromosomes 1D and 3D, and that of the line K14-547-1 had 26A/B and 
      14R chromosomes, plus one pair of centric 6BL/2DS translocations. This finding 
      implies that some of D genome chromosomes can be spontaneously and stably 
      incorporated into the hexaploid triticale. Additionally, a variety of 
      high-molecular-weight glutenin subunits (HMW-GS) compositions were detected in 
      the six hexaploid triticale lines, respectively. Besides, compared with its 
      recurrent triticale parent Zhongsi828, these lines showed high level of 
      resistance to stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens 
      prevalent in China, including V26/Gui 22. These new hexaploid triticales not only 
      enhanced diversification of triticale but also could be utilized as valuable 
      germplasm for wheat improvement.
FAU - Kang, Houyang
AU  - Kang H
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Wang, Hao
AU  - Wang H
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Huang, Juan
AU  - Huang J
AD  - Dazhou Institute of Agricultural Science, 188 Jianmin Road, Dazhou, 635000, 
      Sichuan, China.
FAU - Wang, Yujie
AU  - Wang Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Li, Daiyan
AU  - Li D
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Diao, Chengdou
AU  - Diao C
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Zhu, Wei
AU  - Zhu W
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Tang, Yao
AU  - Tang Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Fan, Xing
AU  - Fan X
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Zeng, Jian
AU  - Zeng J
AD  - College of Resources, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, 
      Chengdu, 611130, Sichuan, China.
FAU - Xu, Lili
AU  - Xu L
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Sha, Lina
AU  - Sha L
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Zhang, Haiqin
AU  - Zhang H
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
FAU - Zhou, Yonghong
AU  - Zhou Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, 
      Wenjiang, Chengdu, 611130, Sichuan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160516
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Chromosomes, Plant
MH  - Genome, Plant
MH  - *Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Miosis/genetics
MH  - Poaceae/*genetics
MH  - *Polyploidy
MH  - Secale/*genetics
MH  - Triticale/*genetics
MH  - Triticum/*genetics
PMC - PMC4868327
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/05/18 06:00
MHDA- 2017/07/08 06:00
PMCR- 2016/05/16
CRDT- 2016/05/17 06:00
PHST- 2016/01/14 00:00 [received]
PHST- 2016/05/01 00:00 [accepted]
PHST- 2016/05/17 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/07/08 06:00 [medline]
PHST- 2016/05/16 00:00 [pmc-release]
AID - PONE-D-16-01881 [pii]
AID - 10.1371/journal.pone.0155667 [doi]
PST - epublish
SO  - PLoS One. 2016 May 16;11(5):e0155667. doi: 10.1371/journal.pone.0155667. 
      eCollection 2016.