PMID- 27183608
OWN - NLM
STAT- MEDLINE
DCOM- 20170928
LR  - 20180121
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 196
IP  - 11
DP  - 2016 Jun 1
TI  - TGF-beta Signaling in Dendritic Cells Governs Colonic Homeostasis by Controlling 
      Epithelial Differentiation and the Luminal Microbiota.
PG  - 4603-13
LID - 10.4049/jimmunol.1502548 [doi]
AB  - Dendritic cells (DCs) mediate host immune responses to gut microbes and play 
      critical roles in inflammatory bowel disease. In this study, we examined the role 
      of TGF-beta signaling in DCs in colonic homeostasis. CD11c-cre Tgfbr2(fl/fl) mice 
      developed spontaneous colitis, and CD11c-cre Tgfbr2(fl/+) mice exhibited 
      susceptibility to dextran sulfate sodium-induced colitis. Colitis in these mice 
      was characterized by goblet cell depletion and dysbiosis caused by 
      Enterobacteriaceae enrichment. Wild-type mice gavaged with Enterobacteriaceae 
      from CD11c-cre Tgfbr2(fl/fl) mice feces showed severe colitis after dextran 
      sulfate sodium treatment, whereas those treated with Notch inhibitor exhibited 
      attenuated colonic injury with increased goblet cell numbers, thickened mucus 
      layer, and fewer fecal Enterobacteriaceae Wild-type mice transplanted with 
      CD11c-cre Tgfbr2(fl/fl) bone marrow developed colitis showing increased Jagged1 
      and Jagged2 in DCs, increased Hes1 levels in epithelium, and goblet cell 
      depletion. These findings suggest that TGF-beta signaling in DCs regulates 
      intestinal homeostasis by modulating epithelial cell differentiation and fecal 
      microbiota.
CI  - Copyright (c) 2016 by The American Association of Immunologists, Inc.
FAU - Ihara, Sozaburo
AU  - Ihara S
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Hirata, Yoshihiro
AU  - Hirata Y
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan hiratay-int@h.u-tokyo.ac.jp.
FAU - Serizawa, Takako
AU  - Serizawa T
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Suzuki, Nobumi
AU  - Suzuki N
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Sakitani, Kosuke
AU  - Sakitani K
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Kinoshita, Hiroto
AU  - Kinoshita H
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Hayakawa, Yoku
AU  - Hayakawa Y
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Nakagawa, Hayato
AU  - Nakagawa H
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Ijichi, Hideaki
AU  - Ijichi H
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Tateishi, Keisuke
AU  - Tateishi K
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
FAU - Koike, Kazuhiko
AU  - Koike K
AD  - Department of Gastroenterology, Graduate School of Medicine, The University of 
      Tokyo, Tokyo 113-8655, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160422
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Transforming Growth Factor beta)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Colitis/chemically induced/immunology/microbiology
MH  - Colon/*cytology/immunology/*microbiology
MH  - Dendritic Cells/immunology/*metabolism
MH  - Dextran Sulfate
MH  - Disease Models, Animal
MH  - Epithelial Cells/*cytology
MH  - *Gastrointestinal Microbiome/immunology
MH  - *Homeostasis
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - *Signal Transduction
MH  - Transforming Growth Factor beta/*immunology
EDAT- 2016/05/18 06:00
MHDA- 2017/09/29 06:00
CRDT- 2016/05/17 06:00
PHST- 2015/12/07 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/05/17 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/09/29 06:00 [medline]
AID - jimmunol.1502548 [pii]
AID - 10.4049/jimmunol.1502548 [doi]
PST - ppublish
SO  - J Immunol. 2016 Jun 1;196(11):4603-13. doi: 10.4049/jimmunol.1502548. Epub 2016 
      Apr 22.