PMID- 27183618
OWN - NLM
STAT- MEDLINE
DCOM- 20170928
LR  - 20180121
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 196
IP  - 11
DP  - 2016 Jun 1
TI  - Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung 
      Mucosa of Healthy Humans.
PG  - 4498-509
LID - 10.4049/jimmunol.1600071 [doi]
AB  - Every breath we take contains potentially harmful pathogens or allergens. 
      Dendritic cells (DCs), monocytes, and macrophages are essential in maintaining a 
      delicate balance of initiating immunity without causing collateral damage to the 
      lungs because of an exaggerated inflammatory response. To document the diversity 
      of lung mononuclear phagocytes at steady-state, we performed bronchoscopies on 20 
      healthy subjects, sampling the proximal and distal airways (bronchial wash and 
      bronchoalveolar lavage, respectively), as well as mucosal tissue (endobronchial 
      biopsies). In addition to a substantial population of alveolar macrophages, we 
      identified subpopulations of monocytes, myeloid DCs (MDCs), and plasmacytoid DCs 
      in the lung mucosa. Intermediate monocytes and MDCs were highly frequent in the 
      airways compared with peripheral blood. Strikingly, the density of mononuclear 
      phagocytes increased upon descending the airways. Monocytes from blood and 
      airways produced 10-fold more proinflammatory cytokines than MDCs upon ex vivo 
      stimulation. However, airway monocytes were less inflammatory than blood 
      monocytes, suggesting a more tolerant nature. The findings of this study 
      establish how to identify human lung mononuclear phagocytes and how they function 
      in normal conditions, so that dysregulations in patients with respiratory 
      diseases can be detected to elucidate their contribution to immunity or 
      pathogenesis.
CI  - Copyright (c) 2016 by The American Association of Immunologists, Inc.
FAU - Baharom, Faezzah
AU  - Baharom F
AUID- ORCID: 0000-0003-4513-3600
AD  - Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska 
      Institutet, 171 76 Stockholm, Sweden;
FAU - Thomas, Saskia
AU  - Thomas S
AD  - Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska 
      Institutet, 171 76 Stockholm, Sweden;
FAU - Rankin, Gregory
AU  - Rankin G
AUID- ORCID: 0000-0002-8753-830X
AD  - Division of Medicine, Department of Public Health and Clinical Medicine, Umea 
      University, 901 85 Umea, Sweden; and.
FAU - Lepzien, Rico
AU  - Lepzien R
AD  - Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska 
      Institutet, 171 76 Stockholm, Sweden;
FAU - Pourazar, Jamshid
AU  - Pourazar J
AUID- ORCID: 0000-0003-0041-4644
AD  - Division of Medicine, Department of Public Health and Clinical Medicine, Umea 
      University, 901 85 Umea, Sweden; and.
FAU - Behndig, Annelie F
AU  - Behndig AF
AUID- ORCID: 0000-0002-1533-9002
AD  - Division of Medicine, Department of Public Health and Clinical Medicine, Umea 
      University, 901 85 Umea, Sweden; and.
FAU - Ahlm, Clas
AU  - Ahlm C
AUID- ORCID: 0000-0003-2018-8592
AD  - Department of Clinical Microbiology, Infectious Diseases, Umea University, 901 85 
      Umea, Sweden.
FAU - Blomberg, Anders
AU  - Blomberg A
AUID- ORCID: 0000-0002-2452-7347
AD  - Division of Medicine, Department of Public Health and Clinical Medicine, Umea 
      University, 901 85 Umea, Sweden; and.
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
AD  - Immunology and Allergy Unit, Department of Medicine, Solna, Karolinska 
      Institutet, 171 76 Stockholm, Sweden; anna.smed.sorensen@ki.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160502
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Dendritic Cells/immunology
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Inflammation/*immunology
MH  - Male
MH  - Monocytes/*immunology
MH  - Respiratory Mucosa/*immunology
MH  - Young Adult
EDAT- 2016/05/18 06:00
MHDA- 2017/09/29 06:00
CRDT- 2016/05/17 06:00
PHST- 2016/01/12 00:00 [received]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/05/17 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/09/29 06:00 [medline]
AID - jimmunol.1600071 [pii]
AID - 10.4049/jimmunol.1600071 [doi]
PST - ppublish
SO  - J Immunol. 2016 Jun 1;196(11):4498-509. doi: 10.4049/jimmunol.1600071. Epub 2016 
      May 2.