PMID- 27184816
OWN - NLM
STAT- MEDLINE
DCOM- 20180402
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 May 17
TI  - Induction of C/EBP homologous protein-mediated apoptosis and autophagy by 
      licochalcone A in non-small cell lung cancer cells.
PG  - 26241
LID - 10.1038/srep26241 [doi]
LID - 26241
AB  - Licochalcone A (LCA), a flavonoid isolated from the famous Chinese medicinal herb 
      Glycyrrhiza uralensis Fisch, presents obvious anti-cancer effects. In this study, 
      the anti-cancer effects and potential mechanisms of LCA in non-small cell lung 
      cancer (NSCLC) cells were studied. LCA decreased cell viability, increased 
      lactate dehydrogenase release, and induced apoptosis in a concentration-dependent 
      manner in NSCLC cells while not in human embryonic lung fibroblast cells. The 
      expression of phosphatidylethanolamine-modified microtubule-associated protein 
      light-chain 3 (LC3-II) and formation of GFP-LC3 punta, two autophagic markers, 
      were increased after treatment with LCA. LCA-induced LC3-II expression was 
      increased when combined with chloroquine (CQ), while knock-down of autophagy 
      related protein (ATG) 7 or ATG5 reversed LCA-induced LC3-II expression and 
      GFP-LC3 punta formation, suggesting that LCA induced autophagy in NSCLC cells. 
      Inhibition of autophagy could not reverse the LCA-induced cell viability decrease 
      and apoptosis. In addition, LCA increased the expression of endoplasmic reticulum 
      stress related proteins, such as binding immunoglobulin protein and C/EBP 
      homologous protein (CHOP). Knock-down of CHOP reversed LCA-induced cell viability 
      decrease, apoptosis, and autophagy. Taken together, LCA-induced autophagic effect 
      is an accompanied phenomenon in NSCLC cells, and CHOP is critical for LCA-induced 
      cell viability decrease, apoptosis, and autophagy.
FAU - Tang, Zheng-Hai
AU  - Tang ZH
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Chen, Xin
AU  - Chen X
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Wang, Zhao-Yu
AU  - Wang ZY
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Chai, Ke
AU  - Chai K
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Wang, Ya-Fang
AU  - Wang YF
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Xu, Xiao-Huang
AU  - Xu XH
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Wang, Xiao-Wen
AU  - Wang XW
AD  - Medical Center, Yuquan Hospital, Tsinghua University, Beijing, China.
FAU - Lu, Jia-Hong
AU  - Lu JH
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Wang, Yi-Tao
AU  - Wang YT
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Chen, Xiu-Ping
AU  - Chen XP
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
FAU - Lu, Jin-Jian
AU  - Lu JJ
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macao, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160517
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Chalcones)
RN  - 0 (DDIT3 protein, human)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - JTV5467968 (licochalcone A)
SB  - IM
MH  - A549 Cells
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/drug effects
MH  - Autophagy/drug effects
MH  - Carcinoma, Non-Small-Cell Lung/*drug therapy/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Chalcones/*pharmacology
MH  - Drugs, Chinese Herbal/pharmacology
MH  - Gene Knockdown Techniques
MH  - Glycyrrhiza uralensis/chemistry
MH  - Humans
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lung Neoplasms/*drug therapy/*metabolism/pathology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Transcription Factor CHOP/antagonists & inhibitors/genetics/*metabolism
PMC - PMC4869105
EDAT- 2016/05/18 06:00
MHDA- 2018/04/03 06:00
PMCR- 2016/05/17
CRDT- 2016/05/18 06:00
PHST- 2016/01/08 00:00 [received]
PHST- 2016/04/28 00:00 [accepted]
PHST- 2016/05/18 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2018/04/03 06:00 [medline]
PHST- 2016/05/17 00:00 [pmc-release]
AID - srep26241 [pii]
AID - 10.1038/srep26241 [doi]
PST - epublish
SO  - Sci Rep. 2016 May 17;6:26241. doi: 10.1038/srep26241.