PMID- 27186338
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160517
LR  - 20200930
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 10
IP  - 1
DP  - 2016
TI  - Identification of homogeneously staining regions by G-banding and chromosome 
      microdissection, and FISH marker selection using human Alu sequence primers in a 
      scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria).
PG  - 61-75
LID - 10.3897/CompCytogen.v10i1.5699 [doi]
AB  - Karyotype analysis was performed on the scleractinian coral Coelastrea aspera 
      Verrill, 1866, commonly found along temperate coasts in Japan (30-35 degrees N) and in 
      coastal waters in the Indian and Pacific oceans. G-banding of Coelastrea aspera 
      was successfully performed, although the banding pattern was not as clear as that 
      in mammals. The karyogram clearly revealed that this coral had a homogeneously 
      staining region (hsr) in chromosome 11. This hsr consisted of ribosomal RNA 
      (rRNA) related genes, which was demonstrated by fluorescence in situ 
      hybridization (FISH) with probes generated using 28S ribosomal DNA (rDNA) primers 
      and those generated through chromosome microdissection. In addition, we conducted 
      silver-stained nucleolus organizer region (Ag-NOR) analysis and found Ag 
      depositions in the interphase nuclei but not on rRNA gene loci and hsr(s) in the 
      mitotic stage. The hsr of this coral was observed in approximately 50% of the 
      metaphase spreads analyzed. This may explain the diversity of coral rDNA based on 
      the molecular study of sequence analysis. Furthermore, it was discovered that 
      human telomere and Alu repeated sequences were present in this Coelastrea aspera. 
      Probes derived from human Alu sequences are expected to play an important role in 
      the classification of corals. Overall, our data can be of great value in 
      discriminating among scleractinian coral species and understanding their 
      genetics, including chromosomal evolution.
FAU - Taguchi, Takahiro
AU  - Taguchi T
AD  - Division of Human Health and Medical Science, Graduate School of Kuroshio 
      Science, Kochi University, Nankoku, Kochi 783-8505, Japan.
FAU - Kubota, Satoshi
AU  - Kubota S
AD  - Division of Human Health and Medical Science, Graduate School of Kuroshio 
      Science, Kochi University, Nankoku, Kochi 783-8505, Japan.
FAU - Mezaki, Takuma
AU  - Mezaki T
AD  - Kuroshio Biological Research Foundation, Otsuki, Hata County, Kochi 788-0333, 
      Japan.
FAU - Tagami, Erika
AU  - Tagami E
AD  - Department of Molecular & Cellular Biology, Kochi Medical School, Kochi 
      University.
FAU - Sekida, Satoko
AU  - Sekida S
AD  - Division of Marine Bioresources, Graduate School of Kuroshio Science, Kochi 
      University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan.
FAU - Nakachi, Shu
AU  - Nakachi S
AD  - Kuroshio Biological Research Foundation, Otsuki, Hata County, Kochi 788-0333, 
      Japan.
FAU - Okuda, Kazuo
AU  - Okuda K
AD  - Division of Marine Bioresources, Graduate School of Kuroshio Science, Kochi 
      University, 2-5-1 Akebono-cho, Kochi 780-8520, Japan.
FAU - Tominaga, Akira
AU  - Tominaga A
AD  - Division of Human Health and Medical Science, Graduate School of Kuroshio 
      Science, Kochi University, Nankoku, Kochi 783-8505, Japan; Department of 
      Molecular & Cellular Biology, Kochi Medical School, Kochi University.
LA  - eng
PT  - Journal Article
DEP - 20160122
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC4856926
OTO - NOTNLM
OT  - Alu repeats
OT  - Coral
OT  - FISH
OT  - chromosome microdissection
OT  - hsr
OT  - karyotype
EDAT- 2016/05/18 06:00
MHDA- 2016/05/18 06:01
PMCR- 2016/01/22
CRDT- 2016/05/18 06:00
PHST- 2015/07/20 00:00 [received]
PHST- 2015/10/22 00:00 [accepted]
PHST- 2016/05/18 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2016/05/18 06:01 [medline]
PHST- 2016/01/22 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v10i1.5699 [doi]
PST - epublish
SO  - Comp Cytogenet. 2016 Jan 22;10(1):61-75. doi: 10.3897/CompCytogen.v10i1.5699. 
      eCollection 2016.