PMID- 27186345 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20160517 LR - 20200930 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 10 IP - 1 DP - 2016 TI - Chromosomal mapping of tandem repeats in the Yesso Scallop, Patinopecten yessoensis (Jay, 1857), utilizing fluorescence in situ hybridization. PG - 157-69 LID - 10.3897/CompCytogen.v10i1.7391 [doi] AB - Construction of cytogenetic maps can provide important information for chromosome identification, chromosome evolution and genomic research. However, it hasn't been conducted in many scallop species yet. In the present study, we attempted to map 12 fosmid clones containing tandem repeats by fluorescence in situ hybridization (FISH) in the Yesso scallop Patinopecten yessoensis (Jay, 1857). The results showed 6 fosmid clones were successfully mapped and distributed in 6 different pairs of chromosomes. Three clones were respectively assigned to a pair of metacentric chromosomes, a pair of submetacentric chromosomes and a pair of telocentric chromosomes and the remaining 3 clones showed their loci on three different pairs of subtelocentric chromosomes by co-hybridization. In summary, totally 8 pairs of chromosomes of the Yesso scallop were identified by 6 fosmid clones and two rDNA probes. Furthermore, 6 tandem repeats of 5 clones were sequenced and could be developed as chromosome specific markers for the Yesso scallop. The successful localization of fosmid clones will undoubtedly facilitate the integration of linkage groups with cytogenetic map and genomic research for the Yesso scallop. FAU - Li, Xuan AU - Li X AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. FAU - Yang, Zujing AU - Yang Z AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. FAU - Liao, Huan AU - Liao H AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. FAU - Zhang, Zhengrui AU - Zhang Z AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. FAU - Huang, Xiaoting AU - Huang X AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. FAU - Bao, Zhenmin AU - Bao Z AD - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China. LA - eng PT - Journal Article DEP - 20160321 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC4856933 OTO - NOTNLM OT - FISH OT - Scallop OT - chromosome identification OT - fosmid OT - tandem repeats EDAT- 2016/05/18 06:00 MHDA- 2016/05/18 06:01 PMCR- 2016/03/21 CRDT- 2016/05/18 06:00 PHST- 2015/12/03 00:00 [received] PHST- 2016/01/26 00:00 [accepted] PHST- 2016/05/18 06:00 [entrez] PHST- 2016/05/18 06:00 [pubmed] PHST- 2016/05/18 06:01 [medline] PHST- 2016/03/21 00:00 [pmc-release] AID - 10.3897/CompCytogen.v10i1.7391 [doi] PST - epublish SO - Comp Cytogenet. 2016 Mar 21;10(1):157-69. doi: 10.3897/CompCytogen.v10i1.7391. eCollection 2016.