PMID- 27190536
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160518
LR  - 20200930
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2016
DP  - 2016
TI  - Effects of Zusanli and Ashi Acupoint Electroacupuncture on Repair of Skeletal 
      Muscle and Neuromuscular Junction in a Rabbit Gastrocnemius Contusion Model.
PG  - 7074563
LID - 10.1155/2016/7074563 [doi]
LID - 7074563
AB  - Objective. To explore the effects of electroacupuncture (EA) at ST36 (EA-ST36) 
      and at Ashi acupoints (EA-Ashi) on skeletal muscle repair. Methods. Seventy-five 
      rabbits were randomly divided into five groups: normal, contusion, EA-Ashi, 
      EA-ST36, and EA at Ashi acupoints and ST36 (EA-AS). EA (0.4 mA, 2 Hz, 15 min) was 
      applied after an acute gastrocnemius contusion. The morphology of myofibers and 
      neuromuscular junctions (NMJs) and expressions of growth differentiation factor-8 
      (GDF-8), acetylcholinesterase (AChE), Neuregulin 1 (NGR1), and muscle-specific 
      kinase (MuSK) were assessed 7, 14, and 28 days after contusion. Results. Compared 
      with that in contusion group, there was an increase in the following respective 
      parameters in treatment groups: the number and diameter of myofibers, the mean 
      staining area, and continuities of NMJs. A comparison of EA-Ashi and EA-ST36 
      groups indicated that average myofiber diameter, mean staining area of NMJs, and 
      expressions of AChE and NRG1 were higher in EA-Ashi group, whereas expression of 
      GDF-8 decreased on day 7. However, increases in myofiber numbers, expressions of 
      MuSK and AChE, as well as decreases in GDF-8 expression, and the discontinuities 
      were observed in EA-ST36 group on the 28th day. Conclusion. Both EA-ST36 and 
      EA-Ashi promoted myofiber regeneration and restoration of NMJs. EA-Ashi was more 
      effective at earlier stages, whereas EA-ST36 played a more important role at 
      later stages.
FAU - Yu, Zhan-Ge
AU  - Yu ZG
AD  - Department of Graduate School, Beijing University of Chinese Medicine, Beijing 
      100029, China.
FAU - Wang, Rong-Guo
AU  - Wang RG
AD  - College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing 100029, China.
FAU - Xiao, Cheng
AU  - Xiao C
AD  - Institute of Clinical Medicine, China-Japan Friendship Hospital, Beijing 100029, 
      China.
FAU - Zhao, Jun-Yun
AU  - Zhao JY
AD  - School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 
      100029, China.
FAU - Shen, Qian
AU  - Shen Q
AD  - Department of Tuina, Dongfang Hospital, Beijing University of Chinese Medicine, 
      Beijing 100078, China.
FAU - Liu, Shou-Yao
AU  - Liu SY
AD  - Department of Traditional Chinese Medicine Surgery, China-Japan Friendship 
      Hospital, Beijing 100029, China.
FAU - Xu, Qian-Wei
AU  - Xu QW
AD  - Department of Traditional Chinese Medicine, Northern Hospital, Beijing 100029, 
      China.
FAU - Zhang, Qing-Xi
AU  - Zhang QX
AD  - Department of Graduate School, Peking University of Health Science Center, 
      Beijing 100029, China.
FAU - Wang, Yun-Ting
AU  - Wang YT
AD  - Department of Orthopedics, China-Japan Friendship Hospital, Beijing 100029, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20160413
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC4846758
EDAT- 2016/05/18 06:00
MHDA- 2016/05/18 06:01
PMCR- 2016/04/13
CRDT- 2016/05/19 06:00
PHST- 2016/01/12 00:00 [received]
PHST- 2016/03/07 00:00 [revised]
PHST- 2016/03/16 00:00 [accepted]
PHST- 2016/05/19 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2016/05/18 06:01 [medline]
PHST- 2016/04/13 00:00 [pmc-release]
AID - 10.1155/2016/7074563 [doi]
PST - ppublish
SO  - Evid Based Complement Alternat Med. 2016;2016:7074563. doi: 10.1155/2016/7074563. 
      Epub 2016 Apr 13.