PMID- 27190988
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20181113
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - HSP27 Inhibits Homocysteine-Induced Endothelial Apoptosis by Modulation of ROS 
      Production and Mitochondrial Caspase-Dependent Apoptotic Pathway.
PG  - 4847874
LID - 10.1155/2016/4847874 [doi]
LID - 4847874
AB  - Objectives. Elevated plasma homocysteine (Hcy) could lead to endothelial 
      dysfunction and is viewed as an independent risk factor for atherosclerosis. Heat 
      shock protein 27 (HSP27), a small heat shock protein, is reported to exert 
      protective effect against atherosclerosis. This study aims to investigate the 
      protective effect of HSP27 against Hcy-induced endothelial cell apoptosis in 
      human umbilical vein endothelial cells (HUVECs) and to determine the underlying 
      mechanisms. Methods. Apoptosis, reactive oxygen species (ROS), and mitochondrial 
      membrane potential (MMP) of normal or HSP27-overexpressing HUVECs in the presence 
      of Hcy were analyzed by flow cytometry. The mRNA and protein expression levels 
      were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and 
      western blot. Results. We found that Hcy could induce cell apoptosis with 
      corresponding decrease of nitric oxide (NO) level, increase of endothelin-1 
      (ET-1), intracellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion 
      molecule-1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1) levels, 
      elevation of ROS, and dissipation of MMP. In addition, HSP27 could protect the 
      cell against Hcy-induced apoptosis and inhibit the effect of Hcy on HUVECs. 
      Furthermore, HSP27 could increase the ratio of Bcl-2/Bax and inhibit caspase-3 
      activity. Conclusions. Therefore, we concluded that HSP27 played a protective 
      role against Hcy-induced endothelial apoptosis through modulation of ROS 
      production and the mitochondrial caspase-dependent apoptotic pathway.
FAU - Tian, Xin
AU  - Tian X
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Zhao, Lei
AU  - Zhao L
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Song, Xianjing
AU  - Song X
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Yan, Youyou
AU  - Yan Y
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Liu, Ning
AU  - Liu N
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Li, Tianyi
AU  - Li T
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
FAU - Yan, Bingdi
AU  - Yan B
AD  - Department of Respiratory Medicine, The Second Hospital of Jilin University, 218 
      Ziqiang Street, Changchun 130041, China.
FAU - Liu, Bin
AU  - Liu B
AUID- ORCID: 0000-0003-2017-2160
AD  - Department of Cardiology, The Second Hospital of Jilin University, 218 Ziqiang 
      Street, Changchun 130041, China.
LA  - eng
PT  - Journal Article
DEP - 20160417
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Caspases/*metabolism
MH  - HSP27 Heat-Shock Proteins/*metabolism
MH  - Homocysteine/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells/cytology/drug effects/metabolism
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mitochondria/drug effects/metabolism
MH  - Nitric Oxide/biosynthesis
MH  - Protective Agents/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/*drug effects
PMC - PMC4852127
EDAT- 2016/05/18 06:00
MHDA- 2017/02/14 06:00
PMCR- 2016/04/17
CRDT- 2016/05/19 06:00
PHST- 2016/01/13 00:00 [received]
PHST- 2016/03/18 00:00 [revised]
PHST- 2016/03/23 00:00 [accepted]
PHST- 2016/05/19 06:00 [entrez]
PHST- 2016/05/18 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
PHST- 2016/04/17 00:00 [pmc-release]
AID - 10.1155/2016/4847874 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:4847874. doi: 10.1155/2016/4847874. Epub 2016 Apr 17.