PMID- 2719147
OWN - NLM
STAT- MEDLINE
DCOM- 19890616
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 256
IP  - 5 Pt 2
DP  - 1989 May
TI  - Regional alterations in hexokinase activity within rat brain during dehydration 
      and rehydration.
PG  - R1050-5
AB  - Histochemical localization and photodensitometric quantification of the metabolic 
      enzyme, hexokinase (HK), were used to study changes in brain metabolic activity 
      that occur during the development of (5 days) and recovery from (7 days) 
      dehydration. In water-deprived (WD) rats, HK activity increased after 2 days in 
      the subfornical organ (SFO, 22%), nucleus circularis (NC, 36%), parvo- and 
      magnocellular divisions of the paraventricular nucleus (pPVH, 17%; mPVH, 46%) and 
      supraoptic nucleus (SON, 46%). Activity in SFO declined to control levels at 3 
      days but increased again thereafter. In pPVH, mPVH, and SON, activity was 
      elevated until the end of the experiment. In NC, activity returned to control 
      levels within 2 days of drinking by the rats. In salt-loaded (2% NaCl in water) 
      rats, changes were similar to those of WD rats up to 2 days of dehydration (SFO, 
      25%; NC, 20%; pPVH, 16%; mPVH, 38%; SON, 50%). Activity in SFO and pPVH returned 
      to control levels after 3 days and remained unchanged. In mPVH, SON, and NC, 
      activity remained elevated and declined to control levels when salt-free water 
      was provided. Results confirm that water deprivation is a stronger dehydrating 
      stimulus than salt loading. In addition, metabolic activity, as measured by HK 
      activity, varies daily during periods of dehydration and rehydration. These 
      changes cannot always be predicted from results obtained only at the end of a 
      period of dehydration. It is concluded that it is necessary to study 
      dehydration-induced changes in brain metabolism on a daily basis to more fully 
      understand the roles of discrete brain regions in the regulation of body fluids.
FAU - Krukoff, T L
AU  - Krukoff TL
AD  - Department of Anatomy and Cell Biology, Faculty of Medicine, University of 
      Alberta, Edmonton, Canada.
FAU - Vincent, D H
AU  - Vincent DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 451W47IQ8X (Sodium Chloride)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Brain/*enzymology
MH  - Dehydration/*enzymology/therapy
MH  - *Fluid Therapy
MH  - Hexokinase/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sodium Chloride/pharmacology
MH  - Tissue Distribution
MH  - Water Deprivation/physiology
EDAT- 1989/05/01 00:00
MHDA- 1989/05/01 00:01
CRDT- 1989/05/01 00:00
PHST- 1989/05/01 00:00 [pubmed]
PHST- 1989/05/01 00:01 [medline]
PHST- 1989/05/01 00:00 [entrez]
AID - 10.1152/ajpregu.1989.256.5.R1050 [doi]
PST - ppublish
SO  - Am J Physiol. 1989 May;256(5 Pt 2):R1050-5. doi: 
      10.1152/ajpregu.1989.256.5.R1050.