PMID- 27192907
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20190221
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 30
IP  - 2
DP  - 2016 Jan
TI  - [Expression and significance of p-Stat3 and its downstream gene c-myc in 
      laryngeal squamous cell carcinoma tissue and cell line].
PG  - 123-5, 129
AB  - OBJECTIVE: To detect the expressions of p-Stat3 and c-myc in human laryngeal 
      squamous cell carcinoma (LSCC) tissue and Hep2 cell line, and to find the 
      relationship between them. METHOD: Immunohistochemistry was used to detect the 
      expressions of p-Stat3 and c-myc in 60 cases of LSCC and 30 cases of vocal cord 
      polyp tissue. The protein levels of p-Stat3 and c-myc in Hep2 cell line was 
      determined by immunocytochemistry. Western blotting was used to determine the 
      protein levels of p-Stat3 and c-myc in Hep2 after treating with different 
      concentrations of Stattic. RESULT: The positive rates of p-Stat3 and c-myc were 
      65% and 70% in the LSCC tissue, compared with that in the vocal cord polyp 
      tissue, with significant difference (P < 0.05). The expression of p-Stat3 in LSCC 
      tissue was associated with that of c-myc (r = 0.273, P < 0.05). The protein 
      levels of p-Stat3 and c-myc were detected in the Hep2 cell line. Stattic 
      inhibited Stat3 phosphorylation and c-myc in the Hep2 cell line in a 
      concentration-dependent manner. CONCLUSION: p-Stat and c-myc were up regulated in 
      the tissue of laryngeal squamous cell carcinoma and the Hep2 cell line. Stattic 
      inhibits the constitutively active p-Stat3 signaling pathway, and downregulats 
      the expression of c-myc. The strong constitutive p-Stat3 signaling pathway in 
      LSCC makes p-Stat3 a target for the development of novel therapeutic strategies.
FAU - Liu, Li
AU  - Liu L
FAU - Wang, Ying
AU  - Wang Y
FAU - Sang, Jianzhong
AU  - Sang J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
SB  - IM
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Head and Neck Neoplasms/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-myc/*metabolism
MH  - STAT3 Transcription Factor/*metabolism
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2016/05/20 06:00
MHDA- 2016/07/07 06:00
CRDT- 2016/05/20 06:00
PHST- 2016/05/20 06:00 [entrez]
PHST- 2016/05/20 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2016 Jan;30(2):123-5, 129.