PMID- 27193729 OWN - NLM STAT- MEDLINE DCOM- 20170410 LR - 20170410 IS - 1880-3989 (Electronic) IS - 0388-1350 (Linking) VI - 41 IP - 3 DP - 2016 TI - Potential protective effect of arginine against 4-nitrophenol-induced ovarian damage in rats. PG - 371-81 LID - 10.2131/jts.41.371 [doi] AB - 4-nitrophenol (PNP) is generally regarded as a diesel exhaust particle (DEP). Arginine plays an important role as a new feed additive, possessing highly efficient antioxidant activities. Here we investigated the effects of dietary supplementation with arginine against ovarian damage induced by PNP in rats. A total of thirty-two female rats postnatal day 28 (PND 28) were randomly divided into four groups. Two groups were fed with basal diet or 13 g/kg arginine in diet for 4 weeks, respectively; the other two groups were given PNP (100 mg/kg b.w.) daily by subcutaneous injection for 2 weeks following pretreatment with either basal diet or arginine diet for 2 weeks. The values of body weight gain (BWG), average daily gain (ADG) and percentage weight gain (PWG) upon PNP treatment were significantly reduced than those in other groups. The relative liver weight in the PNP group was significantly decreased compared with the control group. Treatment with PNP significant reduced the number of corpora lutea, although serum 17beta-estradiol (E2) and progesterone (P4) concentrations were unchanged. The morphology of the ovaries in PNP-treated rats displayed necrosis, follicular deformation and granulosa cells irregular arrangement. Moreover, exposure to PNP enhanced production of malondialdehyde (MDA) and hydrogen peroxide (H2O2), and decreased the activities of total superoxide dismutase (T-SOD) and catalase (CAT), and the co-administration of arginine can attenuate the oxidative stress caused by PNP. These results suggest that arginine may have a protective effect against ovarian damage induced by PNP owing to its antioxidant capacity effect. FAU - Xu, Wei-Feng AU - Xu WF AD - College of Animal Science and Technology, Nanjing Agricultural University, China. FAU - Li, Yan-Sen AU - Li YS FAU - Dai, Peng-Yuan AU - Dai PY FAU - Li, Chun-Mei AU - Li CM LA - eng PT - Journal Article PL - Japan TA - J Toxicol Sci JT - The Journal of toxicological sciences JID - 7805798 RN - 0 (Antioxidants) RN - 0 (Biomarkers) RN - 0 (Nitrophenols) RN - 4G7DS2Q64Y (Progesterone) RN - 4TI98Z838E (Estradiol) RN - 4Y8F71G49Q (Malondialdehyde) RN - 94ZLA3W45F (Arginine) RN - BBX060AN9V (Hydrogen Peroxide) RN - EC 1.11.1.6 (Catalase) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - Y92ZL45L4R (4-nitrophenol) SB - IM MH - Animals MH - Antioxidants/*pharmacology MH - Arginine/*pharmacology MH - Biomarkers/metabolism MH - Catalase/blood MH - Cytoprotection MH - Estradiol/blood MH - Female MH - Hydrogen Peroxide/blood MH - Malondialdehyde/blood MH - Necrosis MH - Nitrophenols/*toxicity MH - Ovarian Follicle/drug effects/metabolism/pathology MH - Ovary/*drug effects/metabolism/pathology MH - Oxidative Stress/*drug effects MH - Progesterone/blood MH - Rats, Sprague-Dawley MH - Superoxide Dismutase/blood EDAT- 2016/05/20 06:00 MHDA- 2017/04/11 06:00 CRDT- 2016/05/20 06:00 PHST- 2016/05/20 06:00 [entrez] PHST- 2016/05/20 06:00 [pubmed] PHST- 2017/04/11 06:00 [medline] AID - 10.2131/jts.41.371 [doi] PST - ppublish SO - J Toxicol Sci. 2016;41(3):371-81. doi: 10.2131/jts.41.371.