PMID- 27194739
OWN - NLM
STAT- MEDLINE
DCOM- 20171220
LR  - 20220310
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Print)
IS  - 0022-0957 (Linking)
VI  - 67
IP  - 11
DP  - 2016 May
TI  - New insights into the post-translational modification of multiple 
      phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and 
      monoubiquitination during sorghum seed development and germination.
PG  - 3523-36
LID - 10.1093/jxb/erw186 [doi]
AB  - Phosphoenolpyruvate carboxylase (PEPC; E.C. 4.1.1.31) was characterized in 
      developing and germinating sorghum seeds, focusing on the transcript and 
      polypeptide abundance of multiple plant-type phosphoenolpyruvate carboxylase 
      (PTPC) genes, and the post-translational modification of each isoenzyme by 
      phosphorylation versus monoubiquitination during germination. We observed high 
      levels of SbPPC4 (Sb07g014960) transcripts during early development (stage I), 
      and extensive transcript abundance of SbPPC2 (Sb02g021090) and SbPPC3 
      (Sb04g008720) throughout the entire life cycle of the seed. Although tandem mass 
      spectrometry (MS) analysis of immunopurified PTPC indicated that four different 
      PTPC isoenzymes were expressed in the developing and germinating seeds, SbPPC3 
      was the most abundant isozyme of the developing seed, and of the embryo and the 
      aleurone layer of germinating seeds. In vivo phosphorylation of the different 
      PTPC isoenzymes at their conserved N-terminal seryl phosphorylation site during 
      germination was also established by MS/MS analysis. Furthermore, three of the 
      four isoenzymes were partially monoubiquitinated, with MS/MS pinpointing SbPPC2 
      and SbPPC3 monoubiquitination at the conserved Lys-630 and Lys-624 residues, 
      respectively. Our results demonstrate that monoubiquitination and phosphorylation 
      simultaneously occur in vivo with different PTPC isozymes during seed 
      germination. In addition, we show that PTPC monoubiquitination in germinating 
      sorghum seeds always increases at stage II (emergence of the radicle), is 
      maintained during the aerobic period of rapid cell division and reserve 
      mobilization, and remains relatively constant until stage IV-V when coleoptiles 
      initiate the formation of the photosynthetic tissues.
CI  - (c) The Author 2016. Published by Oxford University Press on behalf of the Society 
      for Experimental Biology.
FAU - Ruiz-Ballesta, Isabel
AU  - Ruiz-Ballesta I
AD  - Departamento de Biologia Vegetal, Facultad de Biologia, Universidad de Sevilla, 
      Avda Reina Mercedes n masculine 6, 41012 Sevilla, Spain.
FAU - Baena, Guillermo
AU  - Baena G
AD  - Departamento de Biologia Vegetal, Facultad de Biologia, Universidad de Sevilla, 
      Avda Reina Mercedes n masculine 6, 41012 Sevilla, Spain.
FAU - Gandullo, Jacinto
AU  - Gandullo J
AD  - Departamento de Biologia Vegetal, Facultad de Biologia, Universidad de Sevilla, 
      Avda Reina Mercedes n masculine 6, 41012 Sevilla, Spain.
FAU - Wang, Liqun
AU  - Wang L
AD  - Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 3888 
      Chenhua Road, Shanghai 201602, China State Key Laboratory of Crop Genetics and 
      Germplasm Enhancement, Soybean Research Institute, Nanjing Agricultural 
      University, Nanjing, Jiangsu 210095, China.
FAU - She, Yi-Min
AU  - She YM
AD  - Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 3888 
      Chenhua Road, Shanghai 201602, China.
FAU - Plaxton, William Charles
AU  - Plaxton WC
AD  - Department of Biology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
FAU - Echevarria, Cristina
AU  - Echevarria C
AD  - Departamento de Biologia Vegetal, Facultad de Biologia, Universidad de Sevilla, 
      Avda Reina Mercedes n masculine 6, 41012 Sevilla, Spain echeva@us.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160518
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Isoenzymes)
RN  - 0 (Plant Proteins)
RN  - EC 4.1.1.31 (Phosphoenolpyruvate Carboxylase)
SB  - IM
MH  - Germination
MH  - Isoenzymes/genetics/metabolism
MH  - Phosphoenolpyruvate Carboxylase/*genetics/metabolism
MH  - Phosphorylation
MH  - Plant Proteins/*genetics/metabolism
MH  - Protein Processing, Post-Translational
MH  - Seeds/enzymology/genetics/growth & development
MH  - Sorghum/enzymology/*genetics/growth & development
MH  - Tandem Mass Spectrometry
MH  - Ubiquitination
PMC - PMC4892742
OTO - NOTNLM
OT  - Germination
OT  - Sorghum bicolor
OT  - monoubiquitination
OT  - phosphoenolpyruvate carboxylase
OT  - phosphorylation
OT  - post-translational modifications
OT  - seed development
OT  - seeds
OT  - tissue-specific gene expression.
EDAT- 2016/05/20 06:00
MHDA- 2017/12/21 06:00
PMCR- 2016/05/18
CRDT- 2016/05/20 06:00
PHST- 2016/05/20 06:00 [entrez]
PHST- 2016/05/20 06:00 [pubmed]
PHST- 2017/12/21 06:00 [medline]
PHST- 2016/05/18 00:00 [pmc-release]
AID - erw186 [pii]
AID - 10.1093/jxb/erw186 [doi]
PST - ppublish
SO  - J Exp Bot. 2016 May;67(11):3523-36. doi: 10.1093/jxb/erw186. Epub 2016 May 18.