PMID- 27195296
OWN - NLM
STAT- MEDLINE
DCOM- 20170222
LR  - 20181202
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - The Possible Roles of Biological Bone Constructed with Peripheral Blood Derived 
      EPCs and BMSCs in Osteogenesis and Angiogenesis.
PG  - 8168943
LID - 10.1155/2016/8168943 [doi]
LID - 8168943
AB  - This study aimed to determine the possible potential of partially deproteinized 
      biologic bone (PDPBB) seeded with bone marrow stromal cells (BMSCs) and 
      endothelial progenitor cells (EPCs) in osteogenesis and angiogenesis. BMSCs and 
      EPCs were isolated, identified, and cocultured in vitro, followed by seeding on 
      the PDPBB. Expression of osteogenesis and vascularization markers was quantified 
      by immunofluorescence (IF) staining, immunohistochemistry (IHC), and quantitive 
      real-time polymerase chain reaction (qRT-PCR). Scanning electron microscope (SEM) 
      was also employed to further evaluate the morphologic alterations of cocultured 
      cells in the biologic bone. Results demonstrated that the coculture system 
      combined with BMSCs and EPCs had significant advantages of (i) upregulating the 
      mRNA expression of VEGF, Osteonectin, Osteopontin, and Collagen Type I and (ii) 
      increasing ALP and OC staining compared to the BMSCs or EPCs only group. 
      Moreover, IHC staining for CD105, CD34, and ZO-1 increased significantly in the 
      implanted PDPBB seeded with coculture system, compared to that of BMSCs or EPCs 
      only, respectively. Summarily, the present data provided evidence that PDPBB 
      seeded with cocultured system possessed favorable cytocompatibility, provided 
      suitable circumstances for different cell growth, and had the potential to 
      provide reconstruction for cases with bone defection by promoting osteogenesis 
      and angiogenesis.
FAU - Wu, Li
AU  - Wu L
AD  - Medical Imaging Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
FAU - Zhao, Xian
AU  - Zhao X
AD  - Plastic Surgery Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
FAU - He, Bo
AU  - He B
AD  - Medical Imaging Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
FAU - Jiang, Jie
AU  - Jiang J
AD  - Medical Imaging Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
FAU - Xie, Xiao-Jie
AU  - Xie XJ
AD  - Medical Imaging Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
FAU - Liu, Liu
AU  - Liu L
AD  - Plastic Surgery Department, The First Affiliated Hospital, Kunming Medical 
      University, Kunming 650032, China.
LA  - eng
PT  - Journal Article
DEP - 20160418
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (RNA, Messenger)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Transplantation
MH  - Bone and Bones/*physiology/ultrastructure
MH  - Cell Separation
MH  - Cell Shape
MH  - Coculture Techniques
MH  - Endothelial Progenitor Cells/*cytology
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microvessels/physiology
MH  - *Neovascularization, Physiologic
MH  - Osteoblasts/metabolism
MH  - Osteocalcin/metabolism
MH  - *Osteogenesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4852345
EDAT- 2016/05/20 06:00
MHDA- 2017/02/23 06:00
PMCR- 2016/04/18
CRDT- 2016/05/20 06:00
PHST- 2015/12/14 00:00 [received]
PHST- 2016/03/07 00:00 [revised]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/05/20 06:00 [entrez]
PHST- 2016/05/20 06:00 [pubmed]
PHST- 2017/02/23 06:00 [medline]
PHST- 2016/04/18 00:00 [pmc-release]
AID - 10.1155/2016/8168943 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:8168943. doi: 10.1155/2016/8168943. Epub 2016 Apr 18.