PMID- 27196894
OWN - NLM
STAT- MEDLINE
DCOM- 20170316
LR  - 20181202
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 5
DP  - 2016 May 17
TI  - A Novel Prostate-Specific Membrane-Antigen (PSMA) Targeted Micelle-Encapsulating 
      Wogonin Inhibits Prostate Cancer Cell Proliferation via Inducing Intrinsic 
      Apoptotic Pathway.
LID - 10.3390/ijms17050676 [doi]
LID - 676
AB  - Prostate cancer (PCa) is a malignant tumor for which there are no effective 
      treatment strategies. In this study, we developed a targeted strategy for 
      prostate-specific membrane-antigen (PSMA)-positive PCa in vitro based on 
      2-(3-((S)-5-amino-1-carboxypentyl)ureido) pentanedioic acid (ACUPA) modified 
      polyethylene glycol (PEG)-Cholesterol micelles containing wogonin (WOG), which 
      was named ACUPA-M-WOG. ACUPA-M-WOG was conventionally prepared using a 
      self-assembling method, which produced stable particle size and zeta potential. 
      Moreover, ACUPA-M-WOG showed good drug encapsulating capacity and drug release 
      profiles. Fluorescence activated cell sorting (FACS) results suggested that ACUPA 
      modified PEG-Cholesterol micelles could effectively enhance the drug uptake on 
      PSMA(+) PCa cells, and the cytotoxicity of ACUPA-M-WOG was stronger than other 
      controls according to in vitro cellular proliferation and apoptosis assays, 
      separately through methyl thiazolyl tetrazolium (MTT) and Annexin V/Propidium 
      Iodide (PI) staining. Finally, the molecular mechanisms of ACUPA-M-WOG's effects 
      on human PSMA(+) PCa were investigated, and were mainly the intrinsic or 
      extrinsic apoptosis signaling pathways. The Western blot results suggested that 
      ACUPA-M-WOG could enhance the WOG-induced apoptosis, which was mainly via the 
      intrinsic signaling pathway rather than the extrinsic signaling pathway. In 
      conclusion, ACUPA-M-WOG was successfully developed for WOG-selective delivery to 
      PSMA(+) PCa cells and had stronger inhibition than free drugs, which might make 
      it an effective strategy for PSMA(+) PCa.
FAU - Zhang, Hailong
AU  - Zhang H
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu 610041, China. hailong6891@163.com.
FAU - Liu, Xiaogang
AU  - Liu X
AD  - Department of Gastroenterology, Hospital of the University of Electronic Science 
      and Technology of China and Sichuan Provincial People's Hospital, Chengdu 610041, 
      China. lxg_sph@163.com.
FAU - Wu, Fengbo
AU  - Wu F
AD  - Department of Pharmacy, West China Hospital, West China Medical School, Sichuan 
      University, Chengdu 610041, China. wufengbo_@163.com.
FAU - Qin, Feifei
AU  - Qin F
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu 610041, China. sklb_qff@sina.com.
FAU - Feng, Ping
AU  - Feng P
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu 610041, China. pfyq@yahoo.com.
AD  - Institute of Clinical Trials, West China Hospital, West China Medical School, 
      Sichuan University, Chengdu 610041, China. pfyq@yahoo.com.
FAU - Xu, Ting
AU  - Xu T
AD  - Department of Pharmacy, West China Hospital, West China Medical School, Sichuan 
      University, Chengdu 610041, China. xt_wch@163.com.
FAU - Li, Xiang
AU  - Li X
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu 610041, China. xiangli.87@scu.edu.cn.
AD  - Department of Urology, West China Hospital, West China Medical School, Sichuan 
      University, Chengdu 610041, China. xiangli.87@scu.edu.cn.
FAU - Yang, Li
AU  - Yang L
AD  - State Key Laboratory of Biotherapy, West China Hospital, West China Medical 
      School, Sichuan University, Chengdu 610041, China. yl.tracy73@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160517
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antigens, Surface)
RN  - 0 (Flavanones)
RN  - 0 (Micelles)
RN  - 0 (Pentanoic Acids)
RN  - 0 (polyethylene glycol-cholesterol)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.4.17.21 (FOLH1 protein, human)
RN  - EC 3.4.17.21 (Glutamate Carboxypeptidase II)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - Antigens, Surface
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cholesterol/analogs & derivatives/chemistry
MH  - Flavanones/chemistry/*pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Glutamate Carboxypeptidase II/*antagonists & inhibitors
MH  - Humans
MH  - Male
MH  - Micelles
MH  - Molecular Targeted Therapy/*methods
MH  - Pentanoic Acids/chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Prostatic Neoplasms/drug therapy/*metabolism
MH  - Signal Transduction/drug effects
PMC - PMC4881502
OTO - NOTNLM
OT  - apoptosis
OT  - prostate cancer
OT  - prostate specific membrane-antigen
OT  - wogonin
EDAT- 2016/05/20 06:00
MHDA- 2017/03/17 06:00
PMCR- 2016/05/01
CRDT- 2016/05/20 06:00
PHST- 2016/03/29 00:00 [received]
PHST- 2016/04/21 00:00 [revised]
PHST- 2016/04/25 00:00 [accepted]
PHST- 2016/05/20 06:00 [entrez]
PHST- 2016/05/20 06:00 [pubmed]
PHST- 2017/03/17 06:00 [medline]
PHST- 2016/05/01 00:00 [pmc-release]
AID - ijms17050676 [pii]
AID - ijms-17-00676 [pii]
AID - 10.3390/ijms17050676 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 May 17;17(5):676. doi: 10.3390/ijms17050676.