PMID- 27199296
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20171124
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 30
IP  - 8
DP  - 2016 Aug
TI  - Dendritic cells primed with a chimeric plasmid containing HIV-1-gag associated 
      with lysosomal-associated protein-1 (LAMP/gag) is a potential therapeutic vaccine 
      against HIV.
PG  - 2970-84
LID - 10.1096/fj.201500059 [doi]
AB  - The decline in number and function of T cells is a hallmark of HIV infection, and 
      preservation or restoration of HIV-specific cellular immune response is a major 
      goal of AIDS treatment. Dendritic cells (DCs) play a key role in the initiation 
      and maintenance of the immune response, and their use as a vaccine vehicle is a 
      promising strategy for enhancing vaccine efficacy. We evaluated the potential of 
      DC-mediated immunization with a DNA vaccine consisting of HIV-1-p55gag (gag, 
      group-specific antigen) associated to lysosomal associated protein (LAMP) 
      sequence (LAMP/gag vaccine). Immunization of mice with mouse DCs transfected with 
      LAMP/gag (Lg-mDCs) stimulated more potent B- and T-cell responses than naked DNA 
      or DCs pulsed with inactivated HIV. Anti-Gag antibody levels were sustained for 
      at least 3 mo after immunization, and recall T-cell responses were also strongly 
      detected at this time point. Human DCs transfected with LAMP/gag (Lg-hDCs) were 
      also activated and able to stimulate greater T-cell response than native 
      gag-transfected DCs. Coculture between Lg-hDCs and T lymphocytes obtained from 
      patients with HIV resulted in upregulation of CD38, CD69, HLA-DR, and granzyme B 
      by CD4(+) and CD8(+) T cells, and increased IFN-gamma and TNF-alpha production. These 
      results indicate that the use of LAMP/gag-DC may be an efficient strategy for 
      enhancing immune function in patients with HIV.-Lucas, C. G. D. O., Matassoli, F. 
      L., Pecanha, L. M. T., Santillo, B. T., Oliveira, L. M. D. S., Oshiro, T. M., 
      Marques, E. T. D. A., Jr., Oxenius, A., de Arruda, L. B. Dendritic cells primed 
      with a chimeric plasmid containing HIV-1-gag associated with lysosomal-associated 
      protein-1 (LAMP/gag) is a potential therapeutic vaccine against HIV.
CI  - (c) FASEB.
FAU - Lucas, Carolina G D O
AU  - Lucas CG
AD  - Departamento de Virologia, Instituto de Microbiologia Paulo de Goes, Universidade 
      Federal do Rio de Janeiro, Rio de Janeiro, Brazil;
FAU - Matassoli, Flavio L
AU  - Matassoli FL
AD  - Departamento de Virologia, Instituto de Microbiologia Paulo de Goes, Universidade 
      Federal do Rio de Janeiro, Rio de Janeiro, Brazil;
FAU - Pecanha, Ligia M T
AU  - Pecanha LM
AD  - Departamento de Imunologia, Instituto de Microbiologia Paulo de Goes, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil;
FAU - Santillo, Bruna Tereso
AU  - Santillo BT
AD  - Laboratorio de Dermatologia e Imunodeficiencias (LIM-56), Departamento de 
      Dermatologia, Escola de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil; 
      Institute of Microbiology, Swiss Federal Institute of Technology, Zurich, 
      Switzerland;
FAU - Oliveira, Luanda Mara da Silva
AU  - Oliveira LM
AD  - Laboratorio de Dermatologia e Imunodeficiencias (LIM-56), Departamento de 
      Dermatologia, Escola de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil;
FAU - Oshiro, Telma Miyuki
AU  - Oshiro TM
AD  - Laboratorio de Dermatologia e Imunodeficiencias (LIM-56), Departamento de 
      Dermatologia, Escola de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil;
FAU - Marques, Ernesto T D A Jr
AU  - Marques ET Jr
AD  - Department of Infectious Diseases and Microbiology, Center for Vaccine Research, 
      Pittsburgh, Pennsylvania, USA; and Department of Virology, Centro de Pesquisas 
      Aggeu Magalhaes (CPqAM), Fundacao Oswaldo Cruz (Fiocruz)-Pernambuco, Recife, 
      Brazil.
FAU - Oxenius, Annette
AU  - Oxenius A
AD  - Institute of Microbiology, Swiss Federal Institute of Technology, Zurich, 
      Switzerland;
FAU - de Arruda, Luciana B
AU  - de Arruda LB
AD  - Departamento de Virologia, Instituto de Microbiologia Paulo de Goes, Universidade 
      Federal do Rio de Janeiro, Rio de Janeiro, Brazil; arruda@micro.ufrj.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160519
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (AIDS Vaccines)
RN  - 0 (Lysosomal-Associated Membrane Protein 1)
RN  - 0 (Protein Precursors)
RN  - 0 (p55 gag precursor protein, Human immunodeficiency virus 1)
SB  - IM
MH  - AIDS Vaccines/*immunology
MH  - Animals
MH  - *Dendritic Cells
MH  - Female
MH  - HIV Infections/*therapy
MH  - Humans
MH  - Immunologic Memory
MH  - Lysosomal-Associated Membrane Protein 1/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plasmids
MH  - Protein Precursors/*physiology
OTO - NOTNLM
OT  - HIV immunotherapy
OT  - T-cell activation
OT  - dendritic cell vaccine
EDAT- 2016/05/21 06:00
MHDA- 2017/08/23 06:00
CRDT- 2016/05/21 06:00
PHST- 2015/08/25 00:00 [received]
PHST- 2016/05/02 00:00 [accepted]
PHST- 2016/05/21 06:00 [entrez]
PHST- 2016/05/21 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
AID - fj.201500059 [pii]
AID - 10.1096/fj.201500059 [doi]
PST - ppublish
SO  - FASEB J. 2016 Aug;30(8):2970-84. doi: 10.1096/fj.201500059. Epub 2016 May 19.