PMID- 27206338 OWN - NLM STAT- MEDLINE DCOM- 20170428 LR - 20180201 IS - 1873-2968 (Electronic) IS - 0006-2952 (Linking) VI - 113 DP - 2016 Aug 1 TI - Beneficial impact of intracerebroventricular fractalkine administration on behavioral and biochemical changes induced by prenatal stress in adult rats: Possible role of NLRP3 inflammasome pathway. PG - 45-56 LID - S0006-2952(16)30081-8 [pii] LID - 10.1016/j.bcp.2016.05.008 [doi] AB - Several lines of evidence indicate that adverse experience in early life may be a triggering factor for pathological inflammatory processes and lead to the development of depression. Fractalkine (CX3CL1), a chemokine, plays an important role not only in the migration, differentiation and proliferation of neuronal and glial cells but also in the regulation of neuronal-microglial signaling and the production of pro-inflammatory factors. In the present study, we examined the impact of a prenatal stress procedure on the expression of fractalkine in the hippocampus and frontal cortex of young and adult male rats. Furthermore, we measured the age-dependent effect of stress during pregnancy on the expression of pro-inflammatory factors IL-1beta, IL-18, TNF-alpha, IL-6, and CCL2 in both brain structures. Next, to illustrate the link between fractalkine signaling and the behavioral and biochemical changes induced by prenatal stress, adult prenatally stressed offspring were injected intracerebroventricularly (icv) with exogenous fractalkine. We reported that prenatal stress leads to long-lasting deficits in fractalkine signaling and enhanced inflammatory activation. The study demonstrates that icv administration of fractalkine attenuates the behavioural changes evoked by prenatal stress procedure in adult animals. Moreover, fractalkine administration, exhibits anti-inflammatory action, mainly in the frontal cortex of adult prenatally stressed rats. The effect of fractalkine is related to inhibition of NLRP3 inflammasome. However, its action on the other members of NOD-like receptor family (NLR) cannot be excluded. These findings provide new in vivo evidence that the behavioral and inflammatory disturbances observed in adult prenatally stressed rats may be related to long-lasting malfunctions in fractalkine signaling. CI - Copyright (c) 2016 Elsevier Inc. All rights reserved. FAU - Slusarczyk, Joanna AU - Slusarczyk J AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Trojan, Ewa AU - Trojan E AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Wydra, Karolina AU - Wydra K AD - Laboratory of Drug Addiction, Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Glombik, Katarzyna AU - Glombik K AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Chamera, Katarzyna AU - Chamera K AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Kucharczyk, Mateusz AU - Kucharczyk M AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Budziszewska, Boguslawa AU - Budziszewska B AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Kubera, Marta AU - Kubera M AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Lason, Wladyslaw AU - Lason W AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Filip, Malgorzata AU - Filip M AD - Laboratory of Drug Addiction, Department of Pharmacology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. FAU - Basta-Kaim, Agnieszka AU - Basta-Kaim A AD - Department of Experimental Neuroendocrinology, Institute of Pharmacology, Polish Academy of Sciences, Smetna 12, 31-343 Krakow, Poland. Electronic address: basta@if-pan.krakow.pl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160517 PL - England TA - Biochem Pharmacol JT - Biochemical pharmacology JID - 0101032 RN - 0 (Chemokine CX3CL1) RN - 0 (Cytokines) RN - 0 (Inflammasomes) RN - 0 (NLR Family, Pyrin Domain-Containing 3 Protein) RN - 0 (Nlrp3 protein, rat) RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - *Behavior, Animal/drug effects MH - Chemokine CX3CL1/administration & dosage/genetics/*metabolism/pharmacology MH - Cytokines/genetics MH - Female MH - Hippocampus/growth & development/immunology/*metabolism MH - Inflammasomes/*metabolism MH - Injections, Intraventricular MH - Male MH - NLR Family, Pyrin Domain-Containing 3 Protein/*metabolism MH - Pregnancy MH - Prenatal Exposure Delayed Effects/immunology/*metabolism/psychology MH - RNA, Messenger/genetics MH - Rats, Sprague-Dawley MH - Signal Transduction MH - Stress, Psychological/complications/immunology/*metabolism OTO - NOTNLM OT - Brain OT - Fractalkine (CX3CL1) OT - Inflammation OT - NLRP3 inflammasome OT - Prenatal stress EDAT- 2016/05/22 06:00 MHDA- 2017/04/30 06:00 CRDT- 2016/05/22 06:00 PHST- 2016/04/12 00:00 [received] PHST- 2016/05/16 00:00 [accepted] PHST- 2016/05/22 06:00 [entrez] PHST- 2016/05/22 06:00 [pubmed] PHST- 2017/04/30 06:00 [medline] AID - S0006-2952(16)30081-8 [pii] AID - 10.1016/j.bcp.2016.05.008 [doi] PST - ppublish SO - Biochem Pharmacol. 2016 Aug 1;113:45-56. doi: 10.1016/j.bcp.2016.05.008. Epub 2016 May 17.