PMID- 27206786 OWN - NLM STAT- MEDLINE DCOM- 20171222 LR - 20220331 IS - 1607-842X (Electronic) IS - 0891-6934 (Linking) VI - 49 IP - 6 DP - 2016 Sep TI - Autoantibody targets in vaccine-associated narcolepsy. PG - 421-433 AB - Narcolepsy is a chronic sleep disorder with a yet unknown cause, but the specific loss of hypocretin-producing neurons together with a strong human leukocyte antigen (HLA) association has led to the hypothesis that autoimmune mechanisms might be involved. Here, we describe an extensive effort to profile autoimmunity repertoires in serum with the aim to find disease-related autoantigens. Initially, 57 serum samples from vaccine-associated and sporadic narcolepsy patients and controls were screened for IgG reactivity towards 10 846 fragments of human proteins using planar microarrays. The discovered differential reactivities were verified on suspension bead arrays in the same sample collection followed by further investigation of 14 antigens in 176 independent samples, including 57 narcolepsy patients. Among these 14 antigens, methyltransferase-like 22 (METTL22) and 5'-nucleotidase cytosolic IA (NT5C1A) were recognized at a higher frequency in narcolepsy patients of both sample sets. Upon sequence analysis of the 14 proteins, polymerase family, member 3 (PARP3), acyl-CoA-binding domain containing 7 (ARID4B), glutaminase 2 (GLS2) and cyclin-dependent kinase-like 1 (CDKL1) were found to contain amino acid sequences with homology to proteins found in the H1N1 vaccine. These findings could become useful elements of further clinical assays that aim towards a better phenotypic understanding of narcolepsy and its triggers. FAU - Haggmark-Manberg, Anna AU - Haggmark-Manberg A AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Zandian, Arash AU - Zandian A AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Forsstrom, Bjorn AU - Forsstrom B AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Khademi, Mohsen AU - Khademi M AD - c Neuroimmunology Unit, Department of Clinical Neuroscience, Karolinska Institutet , Stockholm , Sweden. FAU - Lima Bomfim, Izaura AU - Lima Bomfim I AD - c Neuroimmunology Unit, Department of Clinical Neuroscience, Karolinska Institutet , Stockholm , Sweden. FAU - Hellstrom, Cecilia AU - Hellstrom C AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Arnheim-Dahlstrom, Lisen AU - Arnheim-Dahlstrom L AD - d Department of Medical Epidemiology and Biostatistics , Karolinska Institutet , Stockholm , Sweden. FAU - Hallbook, Tove AU - Hallbook T AD - e Division of Pediatrics , Department of Clinical Sciences, Faculty of Medicine, Lund University , Lund , Sweden. FAU - Darin, Niklas AU - Darin N AD - e Division of Pediatrics , Department of Clinical Sciences, Faculty of Medicine, Lund University , Lund , Sweden. FAU - Lundberg, Ingrid E AU - Lundberg IE AD - f Rheumatology Unit, Department of Medicine, Karolinska University Hospital, Solna, Karolinska Institutet , Stockholm , Sweden. FAU - Uhlen, Mathias AU - Uhlen M AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Partinen, Markku AU - Partinen M AD - g Helsinki Sleep Clinic, Vitalmed Research Centre , Helsinki , Finland , and. AD - h Department of Clinical Neurosciences , University of Helsinki , Helsinki , Finland. FAU - Schwenk, Jochen M AU - Schwenk JM AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. FAU - Olsson, Tomas AU - Olsson T AD - c Neuroimmunology Unit, Department of Clinical Neuroscience, Karolinska Institutet , Stockholm , Sweden. FAU - Nilsson, Peter AU - Nilsson P AD - a Affinity Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. AD - b KTH Center for Applied Proteomics, SciLifeLab, School of Biotechnology, KTH-Royal Institute of Technology , Stockholm , Sweden. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160520 PL - England TA - Autoimmunity JT - Autoimmunity JID - 8900070 RN - 0 (Autoantibodies) RN - 0 (Autoantigens) RN - 0 (Epitopes) RN - 0 (Immunoglobulin G) RN - 0 (Vaccines) RN - EC 3.1.3.5 (5'-Nucleotidase) RN - EC 3.1.3.5 (NT5C1A protein, human) SB - IM MH - 5'-Nucleotidase/immunology MH - Adolescent MH - Adult MH - Aged MH - Autoantibodies/blood/*immunology MH - Autoantigens/immunology MH - *Autoimmunity MH - Case-Control Studies MH - Child MH - Epitope Mapping MH - Epitopes/immunology MH - Female MH - Humans MH - Immunoglobulin G/blood/immunology MH - Male MH - Middle Aged MH - Narcolepsy/*etiology MH - Vaccines/*adverse effects MH - Young Adult OTO - NOTNLM OT - Narcolepsy OT - autoantibody OT - protein microarray OT - serum OT - vaccine-associated EDAT- 2016/05/22 06:00 MHDA- 2017/12/23 06:00 CRDT- 2016/05/22 06:00 PHST- 2016/05/22 06:00 [pubmed] PHST- 2017/12/23 06:00 [medline] PHST- 2016/05/22 06:00 [entrez] AID - 10.1080/08916934.2016.1183655 [doi] PST - ppublish SO - Autoimmunity. 2016 Sep;49(6):421-433. doi: 10.1080/08916934.2016.1183655. Epub 2016 May 20.