PMID- 27208407
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20170726
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 41
DP  - 2016 May
TI  - Curcumin improves LPS-induced preeclampsia-like phenotype in rat by inhibiting 
      the TLR4 signaling pathway.
PG  - 45-52
LID - S0143-4004(16)30039-X [pii]
LID - 10.1016/j.placenta.2016.03.002 [doi]
AB  - INTRODUCTION: Abnormal inflammation mediated by Toll-like receptor 4 (TLR4) 
      signaling pathway contributes to preeclampsia (PE). Because curcumin can inhibit 
      TLR4 signaling pathway, we investigated its effects on a PE rat model. METHODS: 
      Twenty-one pregnant rats were randomly divided into three groups: 1) seven rats 
      were injected 0.5 mug/kg lipopolysaccharide (LPS) on gestational day (GD) 5 to 
      create a PE model (LPS-treated group), 2) seven rats were injected with a similar 
      dosage of LPS and further treated with curcumin (0.36 mg/kg) 
      (LPS-curcumin-treated group), 3) seven rats received saline (control group). 
      Blood pressure and urinary protein level were observed. Immunostaining and 
      periodic acid-Schiff staining of placenta were conducted. TLR4 and downstream 
      Nuclear Factor-kappaB (NF-kappaB) expressions of placenta were analyzed by Western blot 
      and immunohistochemistry. IL-6 and MCP-1 in rat serum and placenta were 
      determined by ELISA and qRT-PCR. RESULTS: Compared to LPS-treated group, 
      LPS-curcumin-treated group had decreased blood pressure and urinary protein 
      level, similar to control group. Furthermore, deficient trophoblast invasion and 
      spiral artery remodeling induced by LPS were improved by curcumin. Increased 
      TLR4, NF-kappaB and IL-6, MCP-1 protein expressions in LPS-treated group were 
      significantly decreased after curcumin administration. DISCUSSION: Curcumin 
      improves the PE-like phenotype in rat model by reducing abnormal inflammation 
      related to TLR4 signaling pathway.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Gong, Ping
AU  - Gong P
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital Clinical 
      College of Traditional Chinese and Western Medicine, Nanjing University of 
      Chinese Medicine, Nanjing, China.
FAU - Liu, Mo
AU  - Liu M
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Hong, Guomin
AU  - Hong G
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital Clinical 
      College of Traditional Chinese and Western Medicine, Nanjing University of 
      Chinese Medicine, Nanjing, China.
FAU - Li, Yujing
AU  - Li Y
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Xue, Pingping
AU  - Xue P
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Zheng, Mingming
AU  - Zheng M
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Wu, Mengfei
AU  - Wu M
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Shen, Li
AU  - Shen L
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Yang, Muyi
AU  - Yang M
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China.
FAU - Diao, Zhenyu
AU  - Diao Z
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing 
      University Medical School, Nanjing, China. Electronic address: 
      diaozy1999@tom.com.
FAU - Hu, Yali
AU  - Hu Y
AD  - Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital Clinical 
      College of Traditional Chinese and Western Medicine, Nanjing University of 
      Chinese Medicine, Nanjing, China. Electronic address: gp8858@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160310
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 4)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Chemokine CCL2/blood/genetics
MH  - Curcumin/pharmacology/*therapeutic use
MH  - Female
MH  - Interleukin-6/blood/genetics
MH  - Lipopolysaccharides/*pharmacology
MH  - NF-kappa B/analysis
MH  - Phenotype
MH  - Pre-Eclampsia/chemically induced/*drug therapy
MH  - Pregnancy
MH  - Proteinuria/drug therapy
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/*drug effects
MH  - Toll-Like Receptor 4/analysis/*antagonists & inhibitors
OTO - NOTNLM
OT  - Curcumin
OT  - Lipopolysaccharide
OT  - Preeclampsia
OT  - Spiral artery remodeling
OT  - Toll-like receptor 4
EDAT- 2016/05/22 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/05/22 06:00
PHST- 2015/09/03 00:00 [received]
PHST- 2016/02/06 00:00 [revised]
PHST- 2016/03/01 00:00 [accepted]
PHST- 2016/05/22 06:00 [entrez]
PHST- 2016/05/22 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
AID - S0143-4004(16)30039-X [pii]
AID - 10.1016/j.placenta.2016.03.002 [doi]
PST - ppublish
SO  - Placenta. 2016 May;41:45-52. doi: 10.1016/j.placenta.2016.03.002. Epub 2016 Mar 
      10.