PMID- 27208621
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20220331
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 433
DP  - 2016 Sep 15
TI  - Maternal testosterone and placental function: Effect of electroacupuncture on 
      placental expression of angiogenic markers and fetal growth.
PG  - 1-11
LID - S0303-7207(16)30163-0 [pii]
LID - 10.1016/j.mce.2016.05.014 [doi]
AB  - Women with polycystic ovary syndrome (PCOS) have elevated circulating androgens 
      during pregnancy and are at an increased risk of adverse pregnancy outcomes. Here 
      we tested the hypotheses that maternal androgen excess decrease placental and 
      fetal growth, and placental expression of markers of steroidogenesis, 
      angiogenesis and sympathetic activity, and that acupuncture with low-frequency 
      electrical stimulation prevents these changes. Pregnant rats were exposed to 
      vehicle or testosterone on gestational day (GD)15-19. Low-frequency 
      electroacupuncture (EA) or handling, as a control for the EA procedure, was given 
      to control or testosterone exposed dams on GD16-20. On GD21, blood pressure was 
      measured and maternal blood, fetuses and placentas collected. Placental steroid 
      receptor expression and proteins involved in angiogenic, neurotrophic and 
      adrenergic signaling were analyzed. EA did not affect any variables in control 
      rats except maternal serum corticosterone, which was reduced. EA in testosterone 
      exposed dams compared with controls increased systolic pressure by 30%, decreased 
      circulating norepinephrine and corticosterone, fetal and placental weight and 
      placental VEGFR1 and proNGF protein expression, and increased the VEGFA/VEGFR1 
      ratio, mature NGF (mNGF) and the mNGF/proNGF ratio. In conclusion, low-frequency 
      EA in control animals did not have any negative influence on any of the studied 
      variables. In contrast, EA in pregnant dams exposed to testosterone increased 
      blood pressure and impaired placental growth and function, leading to decreased 
      fetal growth.
CI  - Copyright (c) 2016. Published by Elsevier Ireland Ltd.
FAU - Fornes, Romina
AU  - Fornes R
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, 17177 
      Stockholm, Sweden.
FAU - Hu, Min
AU  - Hu M
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, 17177 
      Stockholm, Sweden.
FAU - Maliqueo, Manuel
AU  - Maliqueo M
AD  - Endocrinology and Metabolism Laboratory, Department of Medicine, West division, 
      University of Chile, Santiago, Chile.
FAU - Kokosar, Milana
AU  - Kokosar M
AD  - Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska 
      Academy, University of Gothenburg, 405 30 Gothenburg, Sweden.
FAU - Benrick, Anna
AU  - Benrick A
AD  - Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska 
      Academy, University of Gothenburg, 405 30 Gothenburg, Sweden.
FAU - Carr, David
AU  - Carr D
AD  - Department of Maternal and Fetal Medicine, UCL Institute for Women's Health, 
      University College London, UK.
FAU - Billig, Hakan
AU  - Billig H
AD  - Institute of Neuroscience and Physiology, Department of Physiology, Sahlgrenska 
      Academy, University of Gothenburg, 405 30 Gothenburg, Sweden.
FAU - Jansson, Thomas
AU  - Jansson T
AD  - Department of Obstetrics & Gynecology, Division of Reproductive Sciences, 
      University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
FAU - Manni, Luigi
AU  - Manni L
AD  - Institute of Translational Pharmacology - CNR, via del Fosso del Cavaliere 100, 
      00133 Rome, Italy.
FAU - Stener-Victorin, Elisabet
AU  - Stener-Victorin E
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, 17177 
      Stockholm, Sweden. Electronic address: elisabet.stener-victorin@ki.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160518
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Biomarkers)
RN  - 3XMK78S47O (Testosterone)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Biomarkers/*metabolism
MH  - Blood Pressure/physiology
MH  - Corticosterone/metabolism
MH  - Electroacupuncture/*adverse effects
MH  - Female
MH  - Fetal Development/*physiology
MH  - Fetus/metabolism/physiology
MH  - Placenta/*metabolism
MH  - Polycystic Ovary Syndrome/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Testosterone/*metabolism
OTO - NOTNLM
OT  - Acupuncture
OT  - Electrical stimulation
OT  - Fetus
OT  - Maternal androgen excess
OT  - Placenta
OT  - Polycystic ovary syndrome
EDAT- 2016/05/22 06:00
MHDA- 2017/11/01 06:00
CRDT- 2016/05/22 06:00
PHST- 2016/02/18 00:00 [received]
PHST- 2016/05/10 00:00 [revised]
PHST- 2016/05/17 00:00 [accepted]
PHST- 2016/05/22 06:00 [entrez]
PHST- 2016/05/22 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
AID - S0303-7207(16)30163-0 [pii]
AID - 10.1016/j.mce.2016.05.014 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2016 Sep 15;433:1-11. doi: 10.1016/j.mce.2016.05.014. Epub 
      2016 May 18.