PMID- 27208783
OWN - NLM
STAT- MEDLINE
DCOM- 20180122
LR  - 20190318
IS  - 1873-6750 (Electronic)
IS  - 0160-4120 (Linking)
VI  - 94
DP  - 2016 Sep
TI  - Assessment of the endocrine-disrupting effects of short-chain chlorinated 
      paraffins in in vitro models.
PG  - 43-50
LID - S0160-4120(16)30180-5 [pii]
LID - 10.1016/j.envint.2016.05.007 [doi]
AB  - Short-chain chlorinated paraffins (SCCPs), which are candidate persistent organic 
      pollutants (POPs) according to the Stockholm Convention, are of great concern 
      because of their persistent bioaccumulation, long-range transport and potential 
      adverse health effects. However, data on the endocrine-disrupting effects of 
      SCCPs remain scarce. In this study, we first adopted two in vitro models 
      (reporter gene assays and H295R cell line) to investigate the 
      endocrine-disrupting effects of three SCCPs (C10-40.40%, C10-66.10% and 
      C11-43.20%) via receptor mediated and non-receptor mediated pathway. The 
      dual-luciferase reporter gene assay revealed that all test chemicals 
      significantly induced estrogenic effects, which were mediated by estrogen 
      receptor alpha (ERalpha), in the following order: C11-43.20%>C10-66.10%>C10-40.40%. 
      Notably, C10-40.40% and C10-66.10% also demonstrated remarkable anti-estrogenic 
      activities. Only C11-43.20% showed glucocorticoid receptor-mediated (GR) 
      antagonistic activity, with a RIC20 value of 2.6x10(-8)mol/L. None of the SCCPs 
      showed any agonistic or antagonistic activities against thyroid receptor beta (TRbeta). 
      Meanwhile, all test SCCPs stimulated the secretion of 17beta-estradiol (E2). Both 
      C10-66.10% and C11-43.20% increased the production of cortisol at a high level in 
      H295R cell lines. In order to explore the possible mechanism underlying the 
      endocrine-disrupting effects of SCCPs through the non-receptor pathway, the mRNA 
      levels of 9 steroidogenic genes were measured by real-time polymerase chain 
      reaction (RT-PCR). StAR, 17betaHSD, CYP11A1, CYP11B1, CYP19 and CYP21 were 
      upregulated in a concentration-dependent manner by all chemicals. The data 
      provided here emphasized that comprehensive assessments of the health and 
      ecological risks of emerging contaminants, such as SCCPs, are of great concern 
      and should be investigated further.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Zhang, Quan
AU  - Zhang Q
AD  - College of Environment, Zhejiang University of Technology, Hangzhou 310032, 
      China; College of Environment & Resource Sciences, Zhejiang University, Hangzhou 
      310058, China.
FAU - Wang, Jinghua
AU  - Wang J
AD  - College of Environment, Zhejiang University of Technology, Hangzhou 310032, 
      China.
FAU - Zhu, Jianqiang
AU  - Zhu J
AD  - College of Environment, Zhejiang University of Technology, Hangzhou 310032, 
      China.
FAU - Liu, Jing
AU  - Liu J
AD  - College of Environment & Resource Sciences, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Zhang, Jianyun
AU  - Zhang J
AD  - College of Environment & Resource Sciences, Zhejiang University, Hangzhou 310058, 
      China.
FAU - Zhao, Meirong
AU  - Zhao M
AD  - College of Environment, Zhejiang University of Technology, Hangzhou 310032, 
      China. Electronic address: zhaomr@zjut.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160518
PL  - Netherlands
TA  - Environ Int
JT  - Environment international
JID - 7807270
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Thyroid Hormone Receptors beta)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 4TI98Z838E (Estradiol)
RN  - 8002-74-2 (Paraffin)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/genetics
MH  - Animals
MH  - CHO Cells
MH  - Cell Line, Tumor
MH  - Cricetulus
MH  - Cytochrome P-450 Enzyme System/genetics
MH  - Endocrine Disruptors/*toxicity
MH  - Environmental Pollutants/*toxicity
MH  - Estradiol/metabolism
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - Genes, Reporter
MH  - Humans
MH  - Hydrocarbons, Chlorinated/*toxicity
MH  - Paraffin/*toxicity
MH  - Phosphoproteins/genetics
MH  - Receptors, Glucocorticoid/antagonists & inhibitors
MH  - Thyroid Hormone Receptors beta/genetics
OTO - NOTNLM
OT  - *Endocrine-disrupting effects
OT  - *Estrogen receptor alpha
OT  - *Glucocorticoid receptor
OT  - *H295R cell line
OT  - *Short-chain chlorinated paraffins
OT  - *Thyroid receptor beta
EDAT- 2016/05/22 06:00
MHDA- 2018/01/23 06:00
CRDT- 2016/05/22 06:00
PHST- 2016/02/29 00:00 [received]
PHST- 2016/04/18 00:00 [revised]
PHST- 2016/05/07 00:00 [accepted]
PHST- 2016/05/22 06:00 [entrez]
PHST- 2016/05/22 06:00 [pubmed]
PHST- 2018/01/23 06:00 [medline]
AID - S0160-4120(16)30180-5 [pii]
AID - 10.1016/j.envint.2016.05.007 [doi]
PST - ppublish
SO  - Environ Int. 2016 Sep;94:43-50. doi: 10.1016/j.envint.2016.05.007. Epub 2016 May 
      18.