PMID- 27211665 OWN - NLM STAT- MEDLINE DCOM- 20170210 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 210 DP - 2016 Nov 1 TI - Application of terahertz spectroscopy imaging for discrimination of transgenic rice seeds with chemometrics. PG - 415-21 LID - S0308-8146(16)30645-8 [pii] LID - 10.1016/j.foodchem.2016.04.117 [doi] AB - Discrimination of genetically modified organisms is increasingly demanded by legislation and consumers worldwide. The feasibility of a non-destructive discrimination of transgenic rice seeds from its non-transgenic counterparts was examined by terahertz spectroscopy imaging system combined with chemometrics. Principal component analysis (PCA), least squares support vector machines (LS-SVM), PCA-back propagation neural network (PCA-BPNN), and random forest (RF) models with the first and second derivative and standard normal variate transformation (SNV) pre-treatments were applied to classify rice seeds based on genotype. The results demonstrated that differences between non-transgenic and transgenic rice seeds did exist, and an excellent classification (accuracy was 96.67% in the prediction set) could be achieved using the RF model combined with the first derivative pre-treatment. The results indicated that THz spectroscopy imaging together with chemometrics would be a promising technique to identify transgenic rice seeds with high efficiency and without any sample preparation. CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Liu, Wei AU - Liu W AD - School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; Intelligent Control and Compute Vision Lab, Hefei University, Hefei 230601, China. FAU - Liu, Changhong AU - Liu C AD - School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. FAU - Hu, Xiaohua AU - Hu X AD - School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. FAU - Yang, Jianbo AU - Yang J AD - Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China. Electronic address: yjianbo@263.net. FAU - Zheng, Lei AU - Zheng L AD - School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China; School of Medical Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: lzheng@hfut.edu.cn. LA - eng PT - Journal Article DEP - 20160426 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM MH - Oryza/*chemistry MH - Seeds/*chemistry MH - Terahertz Spectroscopy/*methods OTO - NOTNLM OT - Chemometrics OT - Non-destructive determination OT - Rice seed OT - Terahertz spectroscopy imaging OT - Transgenic EDAT- 2016/05/24 06:00 MHDA- 2017/02/12 06:00 CRDT- 2016/05/24 06:00 PHST- 2015/12/13 00:00 [received] PHST- 2016/04/24 00:00 [revised] PHST- 2016/04/25 00:00 [accepted] PHST- 2016/05/24 06:00 [entrez] PHST- 2016/05/24 06:00 [pubmed] PHST- 2017/02/12 06:00 [medline] AID - S0308-8146(16)30645-8 [pii] AID - 10.1016/j.foodchem.2016.04.117 [doi] PST - ppublish SO - Food Chem. 2016 Nov 1;210:415-21. doi: 10.1016/j.foodchem.2016.04.117. Epub 2016 Apr 26.