PMID- 27211891
OWN - NLM
STAT- MEDLINE
DCOM- 20180207
LR  - 20190111
IS  - 1477-0539 (Electronic)
IS  - 1477-0520 (Print)
IS  - 1477-0520 (Linking)
VI  - 14
IP  - 23
DP  - 2016 Jun 21
TI  - Synthesis of tumor necrosis factor alpha for use as a mirror-image phage display 
      target.
PG  - 5298-303
LID - 10.1039/c6ob00824k [doi]
AB  - Tumor Necrosis Factor alpha (TNFalpha) is an inflammatory cytokine that plays a 
      central role in the pathogenesis of chronic inflammatory disease. Here we 
      describe the chemical synthesis of l-TNFalpha along with the mirror-image d-protein 
      for use as a phage display target. The synthetic strategy utilized native 
      chemical ligation and desulfurization to unite three peptide segments, followed 
      by oxidative folding to assemble the 52 kDa homotrimeric protein. This synthesis 
      represents the foundational step for discovering an inhibitory d-peptide with the 
      potential to improve current anti-TNFalpha therapeutic strategies.
FAU - Petersen, Mark E
AU  - Petersen ME
AD  - Department of Biochemistry, University of Utah, 15 N Medical Drive East, Rm 4100, 
      Salt Lake City, Utah 84112-5650, USA. Kay@biochem.utah.edu.
FAU - Jacobsen, Michael T
AU  - Jacobsen MT
FAU - Kay, Michael S
AU  - Kay MS
LA  - eng
GR  - F31 CA171677/CA/NCI NIH HHS/United States
GR  - R01 AI076168/AI/NIAID NIH HHS/United States
GR  - T32 HL007576/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20160523
PL  - England
TA  - Org Biomol Chem
JT  - Organic & biomolecular chemistry
JID - 101154995
RN  - 0 (Peptide Library)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Models, Molecular
MH  - *Peptide Library
MH  - Protein Structure, Secondary
MH  - Stereoisomerism
MH  - Tumor Necrosis Factor-alpha/*chemical synthesis/*chemistry
PMC - PMC4900166
MID - NIHMS789374
EDAT- 2016/05/24 06:00
MHDA- 2018/02/08 06:00
PMCR- 2017/06/21
CRDT- 2016/05/24 06:00
PHST- 2016/05/24 06:00 [entrez]
PHST- 2016/05/24 06:00 [pubmed]
PHST- 2018/02/08 06:00 [medline]
PHST- 2017/06/21 00:00 [pmc-release]
AID - 10.1039/c6ob00824k [doi]
PST - ppublish
SO  - Org Biomol Chem. 2016 Jun 21;14(23):5298-303. doi: 10.1039/c6ob00824k. Epub 2016 
      May 23.