PMID- 27212205
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20220409
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 433
DP  - 2016 Sep 15
TI  - Hypoglycemic effect of D-chiro-inositol in type 2 diabetes mellitus rats through 
      the PI3K/Akt signaling pathway.
PG  - 26-34
LID - S0303-7207(16)30162-9 [pii]
LID - 10.1016/j.mce.2016.05.013 [doi]
AB  - In this investigation, a model of type 2 diabetes mellitus (T2DM) was used on 
      Sprague-Dawley (SD) rats to clarify more details of the mechanism in the therapy 
      of T2DM. D-chiro-inositol (DCI) was administrated to the diabetic rats as two 
      doses [30, 60 mg/(kg.body weight.day)]. The biochemical indices revealed that DCI 
      had a positive effect on hypoglycemic activity and promoted the glycogen 
      synthesis. The rats in DCI high-dosage group had a blood glucose reduction rate 
      of 21.5% after 5 weeks of treatment, and had insulin content in serum about 
      15.3 +/- 2.37 mIU/L which was significantly decreased than diabetes control group. 
      Real-time polymerase chain reaction (RT-PCR) results revealed that DCI gave a 
      positive regulation on glycogen synthase (GS) and protein glucose transporter-4 
      (Glut4). Western blotting suggested that DCI could up-regulated the expression of 
      the phosphatidylinositol-3-kinase (PI3K) p85, PI3Kp110, GS as well as the 
      phosphorylation of protein kinase B (Akt) both in the liver and the skeletal 
      muscle. The results also revealed that DCI enhanced the Glut4 expression on 
      skeletal muscle. Above all, DCI played a positive role in regulating 
      insulin-mediated glucose uptake through the PI3K/Akt signaling pathway in T2DM 
      rats.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Gao, Yun-Feng
AU  - Gao YF
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Zhang, Meng-Na
AU  - Zhang MN
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Wang, Tian-Xin
AU  - Wang TX
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Wu, Tian-Chen
AU  - Wu TC
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Ai, Ru-Dan
AU  - Ai RD
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China.
FAU - Zhang, Ze-Sheng
AU  - Zhang ZS
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of 
      Food Engineering and Biotechnology, Tianjin University of Science & Technology, 
      Tianjin 300457, China. Electronic address: zhangzesheng@tust.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160520
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Blood Glucose)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 4L6452S749 (Inositol)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Diabetes Mellitus, Experimental/*drug therapy
MH  - Diabetes Mellitus, Type 2/*drug therapy/metabolism
MH  - Glucose Transporter Type 4/metabolism
MH  - Hypoglycemic Agents/*pharmacology
MH  - Inositol/*pharmacology
MH  - Insulin/metabolism
MH  - Insulin Resistance/physiology
MH  - Male
MH  - Muscle, Skeletal/drug effects/metabolism
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - D-chiro-inositol
OT  - Diabetes mellitus
OT  - Liver tissue
OT  - PI3K/Akt signaling pathway
OT  - Skeletal muscle
EDAT- 2016/05/24 06:00
MHDA- 2017/11/01 06:00
CRDT- 2016/05/24 06:00
PHST- 2016/03/05 00:00 [received]
PHST- 2016/04/13 00:00 [revised]
PHST- 2016/05/15 00:00 [accepted]
PHST- 2016/05/24 06:00 [entrez]
PHST- 2016/05/24 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
AID - S0303-7207(16)30162-9 [pii]
AID - 10.1016/j.mce.2016.05.013 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2016 Sep 15;433:26-34. doi: 10.1016/j.mce.2016.05.013. Epub 
      2016 May 20.