PMID- 27213305
OWN - NLM
STAT- MEDLINE
DCOM- 20170417
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 21
IP  - 5
DP  - 2016 May 18
TI  - Low-Molecular Weight Polyethylenimine Modified with Pluronic 123 and RGD- or 
      Chimeric RGD-NLS Peptide: Characteristics and Transfection Efficacy of Their 
      Complexes with Plasmid DNA.
LID - 10.3390/molecules21050655 [doi]
LID - 655
AB  - To solve the problem of transfection efficiency vs. cytotoxicity and 
      tumor-targeting ability when polyethylenimine (PEI) was used as a nonviral gene 
      delivery vector, new degradable PEI polymers were synthesized via cross-linking 
      low-molecular-weight PEI with Pluronic P123 and then further coupled with a 
      targeting peptide R4 (RGD) and a bifunctional R11 (RGD-NLS), which were termed as 
      P123-PEI-R4 and P123-PEI-R11, respectively. Agarose gel electrophoresis showed 
      that both P123-PEI-R4 and P123-PEI-R11 efficaciously condense plasmid DNA at a 
      polymer-to-pDNA w/w ratio of 3.0 and 0.4, respectively. The polyplexes were 
      stable in the presence of serum and could protect plasmid DNA against DNaseI. 
      They had uniform spherical nanoparticles with appropriate sizes around 100-280 nm 
      and zeta-potentials about +40 mV. Furthermore, in vitro experiments showed that 
      these polyplexes had lower cytotoxicity at any concentration compared with PEI 25 
      kDa, thus giving promise to high transfection efficiency as compared with another 
      P123-PEI derivate conjugated with trifunctional peptide RGD-TAT-NLS 
      (P123-PEI-R18). More importantly, compared with the other polymers, P123-PEI-R11 
      showed the highest transfection efficiency with relatively lower cytotoxicity at 
      any concentration, indicating that the new synthetic polymer P123-PEI-R11 could 
      be used as a safe and efficient gene deliver vector.
FAU - Hu, Jing
AU  - Hu J
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. 15821787592@163.com.
FAU - Zhao, Wenfang
AU  - Zhao W
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. wfzhao0504@163.com.
FAU - Liu, Kehai
AU  - Liu K
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. khliu@shou.edu.cn.
FAU - Yu, Qian
AU  - Yu Q
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. 15001970365@163.com.
FAU - Mao, Yuan
AU  - Mao Y
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. 13262975153@163.com.
FAU - Lu, Zeyu
AU  - Lu Z
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. m18701881332@163.com.
FAU - Zhang, Yaguang
AU  - Zhang Y
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. edwarddo@163.com.
FAU - Zhu, Manman
AU  - Zhu M
AD  - Department of Biopharmaceutics, College of Food Science and Technology, Shanghai 
      Ocean University, Shanghai 201306, China. zhu_manman7526@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160518
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Oligopeptides)
RN  - 0 (Polymers)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA
MH  - Electrophoresis, Agar Gel
MH  - *Gene Transfer Techniques
MH  - Genetic Therapy/*methods
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Weight
MH  - Nanoparticles/chemistry/therapeutic use
MH  - Nuclear Localization Signals/chemistry/*genetics/therapeutic use
MH  - Oligopeptides/chemistry/*genetics/therapeutic use
MH  - Plasmids/chemistry/genetics
MH  - Polyethyleneimine/*chemistry/therapeutic use
MH  - Polymers/chemistry/therapeutic use
MH  - Transfection/methods
PMC - PMC6273895
OTO - NOTNLM
OT  - RGD
OT  - nuclear localization signal
OT  - physico-chemical properties
OT  - polycations
OT  - transfection efficiency
COIS- The authors declare no conflict of interest.
EDAT- 2016/05/24 06:00
MHDA- 2017/04/18 06:00
PMCR- 2016/05/18
CRDT- 2016/05/24 06:00
PHST- 2016/02/22 00:00 [received]
PHST- 2016/05/12 00:00 [revised]
PHST- 2016/05/13 00:00 [accepted]
PHST- 2016/05/24 06:00 [entrez]
PHST- 2016/05/24 06:00 [pubmed]
PHST- 2017/04/18 06:00 [medline]
PHST- 2016/05/18 00:00 [pmc-release]
AID - molecules21050655 [pii]
AID - molecules-21-00655 [pii]
AID - 10.3390/molecules21050655 [doi]
PST - epublish
SO  - Molecules. 2016 May 18;21(5):655. doi: 10.3390/molecules21050655.