PMID- 27213339 OWN - NLM STAT- MEDLINE DCOM- 20170315 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 17 IP - 5 DP - 2016 May 18 TI - Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. LID - 10.3390/ijms17050758 [doi] LID - 758 AB - In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways. FAU - Bak, Min Ji AU - Bak MJ AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. bakmj0130@gmail.com. FAU - Truong, Van-Long AU - Truong VL AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. truonglongpro@gmail.com. FAU - Ko, Se-Yeon AU - Ko SY AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. kose91@daum.net. FAU - Nguyen, Xuan Ngan Giang AU - Nguyen XN AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. ngangiang.25590@gmail.com. FAU - Ingkasupart, Pajaree AU - Ingkasupart P AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. pajaree.ing@gmail.com. FAU - Jun, Mira AU - Jun M AD - Department of Food Science and Nutrition, Dong-A University, Busan 49315, Korea. mjun@dau.ac.kr. FAU - Shin, Jin Young AU - Shin JY AD - Chemistry and Environmental Science, City University of New York-Medgar Evers College, New York, NY 11225, USA. jinyoung.shin@njmeadowlands.gov. FAU - Jeong, Woo-Sik AU - Jeong WS AD - Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea. jeongws@inje.ac.kr. LA - eng PT - Journal Article DEP - 20160518 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antioxidants) RN - 0 (Plant Extracts) RN - 0 (Proanthocyanidins) RN - 3K9958V90M (Ethanol) RN - EC 2.6.1.1 (Aspartate Aminotransferases) RN - EC 2.6.1.2 (Alanine Transaminase) SB - IM MH - Alanine Transaminase/metabolism MH - Animals MH - Antioxidants/*administration & dosage/pharmacology MH - Aspartate Aminotransferases/metabolism MH - Cell Survival/drug effects MH - Ethanol/*toxicity MH - Gene Expression Regulation MH - Hep G2 Cells MH - Humans MH - Liver Diseases, Alcoholic/metabolism/*prevention & control MH - MAP Kinase Signaling System/drug effects MH - Plant Extracts/chemistry/pharmacology MH - Proanthocyanidins/*administration & dosage/pharmacology MH - Rats MH - Rats, Sprague-Dawley MH - Vitis/*chemistry PMC - PMC4881579 OTO - NOTNLM OT - CYP2E1 OT - MAPKs OT - alcohol OT - hepatoprotection OT - oxidative stress OT - procyanidins EDAT- 2016/05/24 06:00 MHDA- 2017/03/16 06:00 PMCR- 2016/05/01 CRDT- 2016/05/24 06:00 PHST- 2016/04/12 00:00 [received] PHST- 2016/05/06 00:00 [revised] PHST- 2016/05/12 00:00 [accepted] PHST- 2016/05/24 06:00 [entrez] PHST- 2016/05/24 06:00 [pubmed] PHST- 2017/03/16 06:00 [medline] PHST- 2016/05/01 00:00 [pmc-release] AID - ijms17050758 [pii] AID - ijms-17-00758 [pii] AID - 10.3390/ijms17050758 [doi] PST - epublish SO - Int J Mol Sci. 2016 May 18;17(5):758. doi: 10.3390/ijms17050758.