PMID- 27213692
OWN - NLM
STAT- MEDLINE
DCOM- 20170710
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 5
DP  - 2016
TI  - Transcriptional Analysis of PRRSV-Infected Porcine Dendritic Cell Response to 
      Streptococcus suis Infection Reveals Up-Regulation of Inflammatory-Related Genes 
      Expression.
PG  - e0156019
LID - 10.1371/journal.pone.0156019 [doi]
LID - e0156019
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) is one of the 
      most important swine pathogens and often serves as an entry door for other viral 
      or bacterial pathogens, of which Streptococcus suis is one of the most common. 
      Pre-infection with PRRSV leads to exacerbated disease caused by S. suis 
      infection. Very few studies have assessed the immunological mechanisms underlying 
      this higher susceptibility. Since antigen presenting cells play a major role in 
      the initiation of the immune response, the in vitro transcriptional response of 
      bone marrow-derived dendritic cells (BMDCs) and monocytes in the context of PRRSV 
      and S. suis co-infection was investigated. BMDCs were found to be more permissive 
      than monocytes to PRRSV infection; S. suis phagocytosis by PRRSV-infected BMDCs 
      was found to be impaired, whereas no effect was found on bacterial intracellular 
      survival. Transcription profile analysis, with a major focus on inflammatory 
      genes, following S. suis infection, with and without pre-infection with PRRSV, 
      was then performed. While PRRSV pre-infection had little effect on monocytes 
      response to S. suis infection, a significant expression of several 
      pro-inflammatory molecules was observed in BMDCs pre-infected with PRRSV after a 
      subsequent infection with S. suis. While an additive effect could be observed for 
      CCL4, CCL14, CCL20, and IL-15, a distinct synergistic up-regulatory effect was 
      observed for IL-6, CCL5 and TNF-alpha after co-infection. This increased 
      pro-inflammatory response by DCs could participate in the exacerbation of the 
      disease observed during PRRSV and S. suis co-infection.
FAU - Auray, Gael
AU  - Auray G
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Lachance, Claude
AU  - Lachance C
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Wang, Yingchao
AU  - Wang Y
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Gagnon, Carl A
AU  - Gagnon CA
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Segura, Mariela
AU  - Segura M
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
FAU - Gottschalk, Marcelo
AU  - Gottschalk M
AD  - Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), 
      Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, 
      St-Hyacinthe, Quebec, Canada, J2S 2M2.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160523
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coinfection/*genetics/immunology
MH  - Dendritic Cells/*metabolism
MH  - Disease Susceptibility/immunology
MH  - Host-Pathogen Interactions/genetics/immunology
MH  - Immunity, Cellular/genetics
MH  - Inflammation/*genetics/microbiology/virology
MH  - Inflammation Mediators/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/immunology
MH  - Porcine respiratory and reproductive syndrome virus/pathogenicity
MH  - Streptococcal Infections/*genetics/immunology
MH  - Streptococcus suis/pathogenicity
MH  - Swine/*genetics/immunology/microbiology/virology
MH  - Swine Diseases/*genetics/immunology/microbiology/virology
MH  - Up-Regulation/genetics
PMC - PMC4877111
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/05/24 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/05/23
CRDT- 2016/05/24 06:00
PHST- 2016/01/04 00:00 [received]
PHST- 2016/05/09 00:00 [accepted]
PHST- 2016/05/24 06:00 [entrez]
PHST- 2016/05/24 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/05/23 00:00 [pmc-release]
AID - PONE-D-16-00169 [pii]
AID - 10.1371/journal.pone.0156019 [doi]
PST - epublish
SO  - PLoS One. 2016 May 23;11(5):e0156019. doi: 10.1371/journal.pone.0156019. 
      eCollection 2016.