PMID- 27214651
OWN - NLM
STAT- MEDLINE
DCOM- 20180222
LR  - 20181005
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 99
DP  - 2016 Aug
TI  - Immunomodulatory and angiogenic responses induced by graphene oxide scaffolds in 
      chronic spinal hemisected rats.
PG  - 72-81
LID - S0142-9612(16)30179-X [pii]
LID - 10.1016/j.biomaterials.2016.05.012 [doi]
AB  - Attractive physic-chemical features of graphene oxide (GO) and promising results 
      in vitro with neural cells encourage its exploration for biomedical applications 
      including neural regeneration. Fueled by previous findings at the subacute state, 
      we herein investigate for the first time chronic tissue responses (at 30 days) to 
      3D scaffolds composed of partially reduced GO (rGO) when implanted in the injured 
      rat spinal cord. These studies aim to define fibrotic, inflammatory and 
      angiogenic changes at the lesion site induced by the chronic implantation of 
      these porous structures. Injured animals receiving no scaffolds show badly 
      structured lesion zones and more cavities than those carrying rGO materials, thus 
      pointing out a significant role of the scaffolds in injury stabilization and 
      sealing. Notably, GFAP(+) cells and pro-regenerative macrophages are evident at 
      their interface. Moreover, rGO scaffolds support angiogenesis around and, more 
      importantly, inside their structure, with abundant and functional new blood 
      vessels in whose proximities inside the scaffolds some regenerated neuronal axons 
      are found. On the contrary, lesion areas without rGO scaffolds show a diminished 
      quantity of blood vessels and no axons at all. These findings provide a 
      foundation for the usefulness of graphene-based materials in the design of novel 
      biomaterials for spinal cord repair and encourage further investigation for the 
      understanding of neural tissue responses to this kind of materials in vivo.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Lopez-Dolado, Elisa
AU  - Lopez-Dolado E
AD  - Hospital Nacional de Paraplejicos, Servicio de Salud de Castilla-La Mancha 
      (SESCAM), Finca la Peraleda s/n, 45071, Toledo, Spain.
FAU - Gonzalez-Mayorga, Ankor
AU  - Gonzalez-Mayorga A
AD  - Hospital Nacional de Paraplejicos, Servicio de Salud de Castilla-La Mancha 
      (SESCAM), Finca la Peraleda s/n, 45071, Toledo, Spain.
FAU - Gutierrez, Maria Concepcion
AU  - Gutierrez MC
AD  - Instituto de Ciencia de Materiales de Madrid (ICMM), Consejo Superior de 
      Investigaciones Cientificas (CSIC), C/Sor Juana Ines de la Cruz 3, 28049, Madrid, 
      Spain.
FAU - Serrano, Maria Concepcion
AU  - Serrano MC
AD  - Hospital Nacional de Paraplejicos, Servicio de Salud de Castilla-La Mancha 
      (SESCAM), Finca la Peraleda s/n, 45071, Toledo, Spain. Electronic address: 
      mslopezterradas@sescam.jccm.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160510
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Oxides)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Angiogenesis Inducing Agents/chemistry/*therapeutic use
MH  - Animals
MH  - Axons/metabolism/pathology
MH  - Biocompatible Materials/chemistry/therapeutic use
MH  - Graphite/chemistry/*therapeutic use
MH  - Humans
MH  - Immunomodulation
MH  - Male
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Transmission
MH  - Neurons/*pathology
MH  - Oxides/chemistry/*therapeutic use
MH  - Rats, Wistar
MH  - Regeneration
MH  - Spinal Cord/blood supply/pathology
MH  - Spinal Cord Injuries/immunology/pathology/surgery/*therapy
MH  - Spinal Cord Regeneration
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Angiogenesis
OT  - Graphene oxide
OT  - Macrophage
OT  - Scaffold
OT  - Spinal cord injury
EDAT- 2016/05/24 06:00
MHDA- 2018/02/23 06:00
CRDT- 2016/05/24 06:00
PHST- 2016/03/21 00:00 [received]
PHST- 2016/05/02 00:00 [revised]
PHST- 2016/05/06 00:00 [accepted]
PHST- 2016/05/24 06:00 [entrez]
PHST- 2016/05/24 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
AID - S0142-9612(16)30179-X [pii]
AID - 10.1016/j.biomaterials.2016.05.012 [doi]
PST - ppublish
SO  - Biomaterials. 2016 Aug;99:72-81. doi: 10.1016/j.biomaterials.2016.05.012. Epub 
      2016 May 10.