PMID- 27215800
OWN - NLM
STAT- MEDLINE
DCOM- 20170904
LR  - 20180905
IS  - 1607-8438 (Electronic)
IS  - 0300-8207 (Linking)
VI  - 57
IP  - 6
DP  - 2016 Nov
TI  - Periodontal ligament stem/progenitor cells with protein-releasing scaffolds for 
      cementum formation and integration on dentin surface.
PG  - 488-495
AB  - Purpose/Aim: Cementogenesis is a critical step in periodontal tissue regeneration 
      given the essential role of cementum in anchoring teeth to the alveolar bone. 
      This study is designed to achieve integrated cementum formation on the root 
      surfaces of human teeth using growth factor-releasing scaffolds with periodontal 
      ligament stem/progenitor cells (PDLSCs). MATERIALS AND METHODS: Human PDLSCs were 
      sorted by CD146 expression, and characterized using CFU-F assay and induced 
      multi-lineage differentiation. Polycaprolactone scaffolds were fabricated using 
      3D printing, embedded with poly(lactic-co-glycolic acids) (PLGA) microspheres 
      encapsulating connective tissue growth factor (CTGF), bone morphogenetic 
      protein-2 (BMP-2), or bone morphogenetic protein-7 (BMP-7). After removing 
      cementum on human tooth roots, PDLSC-seeded scaffolds were placed on the exposed 
      dentin surface. After 6-week culture with cementogenic/osteogenic medium, 
      cementum formation and integration were evaluated by histomorphometric analysis, 
      immunofluorescence, and qRT-PCR. RESULTS: Periodontal ligament (PDL) cells sorted 
      by CD146 and single-cell clones show a superior clonogenecity and multipotency as 
      compared with heterogeneous populations. After 6 weeks, all the growth 
      factor-delivered groups showed resurfacing of dentin with a newly formed 
      cementum-like layer as compared with control. BMP-2 and BMP-7 showed de novo 
      formation of tissue layers significantly thicker than all the other groups, 
      whereas CTGF and BMP-7 resulted in significantly improved integration on the 
      dentin surface. The de novo mineralized tissue layer seen in BMP-7-treated 
      samples expressed cementum matrix protein 1 (CEMP1). Consistently, BMP-7 showed a 
      significant increase in CEMP1 mRNA expression. CONCLUSION: Our findings represent 
      important progress in stem cell-based cementum regeneration as an essential part 
      of periodontium regeneration.
FAU - Cho, Hankyu
AU  - Cho H
AD  - a Regenerative Engineering Laboratory , Columbia University Medical Center , New 
      York , NY , USA.
FAU - Tarafder, Solaiman
AU  - Tarafder S
AD  - a Regenerative Engineering Laboratory , Columbia University Medical Center , New 
      York , NY , USA.
FAU - Fogge, Michael
AU  - Fogge M
AD  - a Regenerative Engineering Laboratory , Columbia University Medical Center , New 
      York , NY , USA.
FAU - Kao, Kristy
AU  - Kao K
AD  - a Regenerative Engineering Laboratory , Columbia University Medical Center , New 
      York , NY , USA.
FAU - Lee, Chang H
AU  - Lee CH
AD  - a Regenerative Engineering Laboratory , Columbia University Medical Center , New 
      York , NY , USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160523
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (CEMP1 protein, human)
RN  - 0 (Proteins)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Cell Separation
MH  - Clone Cells
MH  - Connective Tissue Growth Factor/*pharmacology
MH  - Dental Cementum/*cytology
MH  - Dentin/*chemistry
MH  - Humans
MH  - Middle Aged
MH  - Multipotent Stem Cells/cytology/drug effects
MH  - Periodontal Ligament/*cytology
MH  - Proteins/metabolism
MH  - Stem Cells/*cytology/drug effects
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Tooth Extraction
MH  - Tooth Root/cytology
MH  - Young Adult
OTO - NOTNLM
OT  - 3D printing
OT  - cementum
OT  - controlled delivery
OT  - periodontal ligament stem cells
OT  - regeneration
EDAT- 2016/11/02 06:00
MHDA- 2017/09/05 06:00
CRDT- 2016/05/25 06:00
PHST- 2016/11/02 06:00 [pubmed]
PHST- 2017/09/05 06:00 [medline]
PHST- 2016/05/25 06:00 [entrez]
AID - 10.1080/03008207.2016.1191478 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2016 Nov;57(6):488-495. doi: 10.1080/03008207.2016.1191478. 
      Epub 2016 May 23.