PMID- 27216084
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20200225
IS  - 1364-3703 (Electronic)
IS  - 1464-6722 (Print)
IS  - 1364-3703 (Linking)
VI  - 18
IP  - 5
DP  - 2017 Jun
TI  - Identification of a Vitis vinifera endo-beta-1,3-glucanase with antimicrobial 
      activity against Plasmopara viticola.
PG  - 708-719
LID - 10.1111/mpp.12431 [doi]
AB  - Inducible plant defences against pathogens are stimulated by infections and 
      comprise several classes of pathogenesis-related (PR) proteins. 
      Endo-beta-1,3-glucanases (EGases) belong to the PR-2 class and their expression is 
      induced by many pathogenic fungi and oomycetes, suggesting that EGases play a 
      role in the hydrolysis of pathogen cell walls. However, reports of a direct 
      effect of EGases on cell walls of plant pathogens are scarce. Here, we 
      characterized three EGases from Vitis vinifera whose expression is induced during 
      infection by Plasmopara viticola, the causal agent of downy mildew. Recombinant 
      proteins were expressed in Escherichia coli. The enzymatic characteristics of 
      these three enzymes were measured in vitro and in planta. A functional assay 
      performed in vitro on germinated P. viticola spores revealed a strong anti-P. 
      viticola activity for EGase3, which strikingly was that with the lowest in vitro 
      catalytic efficiency. To our knowledge, this work shows, for the first time, the 
      direct effect against downy mildew of EGases of the PR-2 family from Vitis.
CI  - (c) 2016 BSPP AND JOHN WILEY & SONS LTD.
FAU - Mestre, Pere
AU  - Mestre P
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
FAU - Arista, Gautier
AU  - Arista G
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
FAU - Piron, Marie-Christine
AU  - Piron MC
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
FAU - Rustenholz, Camille
AU  - Rustenholz C
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
FAU - Ritzenthaler, Christophe
AU  - Ritzenthaler C
AD  - Institut de Biologie Moleculaire des Plantes du CNRS, Universite de Strasbourg, 
      12 rue du General Zimmer, Strasbourg, 67084, France.
FAU - Merdinoglu, Didier
AU  - Merdinoglu D
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
FAU - Chich, Jean-Francois
AU  - Chich JF
AD  - SVQV, INRA, Universite de Strasbourg, Colmar, F-68000, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160724
PL  - England
TA  - Mol Plant Pathol
JT  - Molecular plant pathology
JID - 100954969
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Anti-Infective Agents/metabolism/*pharmacology
MH  - Disease Resistance/genetics/physiology
MH  - Gene Expression Regulation, Plant
MH  - Oomycetes/drug effects/*pathogenicity
MH  - Plant Diseases/microbiology
MH  - Plant Proteins/genetics/metabolism/*pharmacology
MH  - Recombinant Proteins/genetics/metabolism/pharmacology
MH  - Vitis/*enzymology
PMC - PMC6638254
OTO - NOTNLM
OT  - PR-2
OT  - Plasmopara viticola
OT  - Vitis vinifera
OT  - endo-beta-1,3-glucanase
OT  - grapevine
EDAT- 2016/05/25 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/07/24
CRDT- 2016/05/25 06:00
PHST- 2016/05/25 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/05/25 06:00 [entrez]
PHST- 2016/07/24 00:00 [pmc-release]
AID - MPP12431 [pii]
AID - 10.1111/mpp.12431 [doi]
PST - ppublish
SO  - Mol Plant Pathol. 2017 Jun;18(5):708-719. doi: 10.1111/mpp.12431. Epub 2016 Jul 
      24.