PMID- 27216977
OWN - NLM
STAT- MEDLINE
DCOM- 20190128
LR  - 20190128
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1862
IP  - 9
DP  - 2016 Sep
TI  - Novel effects of sphingosylphosphorylcholine on invasion of breast cancer: 
      Involvement of matrix metalloproteinase-3 secretion leading to WNT activation.
PG  - 1533-43
LID - S0925-4439(16)30118-1 [pii]
LID - 10.1016/j.bbadis.2016.05.010 [doi]
AB  - Sphingosylphosphorylcholine (SPC) participates in several cellular processes 
      including metastasis. SPC induces keratin reorganization and regulates the 
      viscoelasticity of metastatic cancer cells including PANC-1 cancer cells leading 
      to enhanced migration and invasion. The role of SPC and the relevant mechanism in 
      invasion of breast cell are as yet unknown. SPC dose-dependently induces invasion 
      of breast cancer cells or breast immortalized cells. Reverse transcription 
      polymerase chain reaction and Western blot analyses of MCF10A and ZR-75-1 cells 
      indicated that SPC induces expression and secretion of matrix metalloproteinase-3 
      (MMP3). From online KMPLOT, relapse free survival is high in patients having low 
      MMP3 expressed basal breast cancer (n=581, p=0.032). UK370106 (MMP3 inhibitor) or 
      gene silencing of MMP3 markedly inhibited the SPC-induced invasion of MCF10A 
      cells. An extracellular signal-regulated kinase (ERK) inhibitor, PD98059, 
      significantly suppressed the secretion and the gelatinolytic activity of MMP3, 
      and invasion in MCF10A cells. Over-expression of ERK1 and ERK2 promoted both the 
      expression and secretion of MMP3. In contrast, gene silencing of ERK1 and ERK2 
      attenuated the secretion of MMP3 in MCF10A cells. The effects of SPC-induced MMP3 
      secretion on beta-catenin and TCF/lymphoid enhancer factor (LEF) promoter activity 
      were examined since MMP3 indirectly activates canonical Wnt signaling. SPC 
      induced translocation of beta-catenin to nucleus and increased TCF/LEF promoter 
      activity. These events were suppressed by UK370106 or PD98059. Wnt inhibitor, 
      FH535 inhibited SPC-induced MMP3 secretion and invasion. Taken together, these 
      results suggest that SPC induces MMP3 expression and secretion via ERK leading to 
      Wnt activation.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Kim, Hyun Ji
AU  - Kim HJ
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea.
FAU - Kang, Gyeoung Jin
AU  - Kang GJ
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea.
FAU - Kim, Eun Ji
AU  - Kim EJ
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea.
FAU - Park, Mi Kyung
AU  - Park MK
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea.
FAU - Byun, Hyun Jung
AU  - Byun HJ
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea.
FAU - Nam, Seungyoon
AU  - Nam S
AD  - Department of Life Sciences, College of BioNano Technology, Gachon University, 
      Sungnam 13120, Republic of Korea.
FAU - Lee, Ho
AU  - Lee H
AD  - National Cancer Center, Goyang-si, Gyeonggi-do 10408, Republic of Korea.
FAU - Lee, Chang Hoon
AU  - Lee CH
AD  - BK21PLUS R-FIND team, College of Pharmacy, Dongguk University, Seoul 04620, 
      Republic of Korea. Electronic address: uatheone@dongguk.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160520
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 
      (3-(((2,2-dimethyl-1-(((2-methoxy-1-phenylethyl)amino)carbonyl)propyl)amino)carbonyl)-6-(3-methyl-4-phenylphenyl)hexanoic 
      acid)
RN  - 0 (Caproates)
RN  - 0 (Flavonoids)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Polycyclic Compounds)
RN  - 10216-23-6 (sphingosine phosphorylcholine)
RN  - 107-73-3 (Phosphorylcholine)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - HG18B9YRS7 (Valine)
RN  - NGZ37HRE42 (Sphingosine)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Breast Neoplasms/*drug therapy/*metabolism/pathology
MH  - Caproates/pharmacology
MH  - Cell Line, Tumor
MH  - Female
MH  - Flavonoids/pharmacology
MH  - Gene Silencing
MH  - Humans
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Matrix Metalloproteinase Inhibitors/pharmacology
MH  - Neoplasm Invasiveness/pathology/physiopathology
MH  - Phosphorylcholine/*analogs & derivatives/metabolism/pharmacology
MH  - Polycyclic Compounds/pharmacology
MH  - Sphingosine/*analogs & derivatives/metabolism/pharmacology
MH  - Valine/analogs & derivatives/pharmacology
MH  - Wnt Signaling Pathway/*drug effects
OTO - NOTNLM
OT  - Breast cancer
OT  - FH535
OT  - MMP3
OT  - Sphingosylphosphorylcholine
OT  - UK370106
OT  - Wnt
EDAT- 2016/05/25 06:00
MHDA- 2019/01/29 06:00
CRDT- 2016/05/25 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/04/27 00:00 [revised]
PHST- 2016/05/18 00:00 [accepted]
PHST- 2016/05/25 06:00 [entrez]
PHST- 2016/05/25 06:00 [pubmed]
PHST- 2019/01/29 06:00 [medline]
AID - S0925-4439(16)30118-1 [pii]
AID - 10.1016/j.bbadis.2016.05.010 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2016 Sep;1862(9):1533-43. doi: 
      10.1016/j.bbadis.2016.05.010. Epub 2016 May 20.