PMID- 27217970
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160524
LR  - 20220729
IS  - 2229-5089 (Print)
IS  - 2153-3539 (Electronic)
VI  - 7
DP  - 2016
TI  - Validation of break-apart and fusion MYC probes using a digital fluorescence in 
      situ hybridization capture and imaging system.
PG  - 20
LID - 10.4103/2153-3539.181764 [doi]
LID - 20
AB  - INTRODUCTION: Detection of MYC translocations using fluorescence in situ 
      hybridization (FISH) is important in the evaluation of lymphomas, in particular, 
      Burkitt lymphoma and diffuse large B-cell lymphoma. Our aim was to validate a 
      digital FISH capture and imaging system for the detection of MYC 8q24 
      translocations using LSI-MYC (a break-apart probe) and MYC 8;14 translocation 
      using IGH-MYC (a fusion probe). MATERIALS AND METHODS: LSI-MYC probe was 
      evaluated using tissue sections from 35 patients. IGH-MYC probe was evaluated 
      using tissue sections from forty patients. Sections were processed for FISH and 
      analyzed using traditional methods. FISH slides were then analyzed using the 
      GenASIs capture and analysis system. RESULTS: Results for LSI-MYC had a high 
      degree of correlation between traditional method of FISH analysis and digital 
      FISH analysis. Results for IGH-MYC had a 100% concordance between traditional 
      method of FISH analysis and digital FISH analysis. CONCLUSION: Annotated whole 
      slide images of H and E and FISH sections can be digitally aligned, so that areas 
      of tumor within a section can be matched and evaluated with a greater degree of 
      accuracy. Images can be archived permanently, providing a means for examining the 
      results retrospectively. Digital FISH imaging of the MYC translocations provides 
      a better diagnostic tool compared to traditional methods for evaluating 
      lymphomas.
FAU - Liew, Michael
AU  - Liew M
AD  - Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake 
      City, UT 84108, USA.
FAU - Rowe, Leslie
AU  - Rowe L
AD  - Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake 
      City, UT 84108, USA.
FAU - Clement, Parker W
AU  - Clement PW
AD  - Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake 
      City, UT 84108, USA; Department of Pathology, University of Utah School of 
      Medicine, Salt Lake City, UT 84132, USA.
FAU - Miles, Rodney R
AU  - Miles RR
AD  - Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake 
      City, UT 84108, USA; Department of Pathology, University of Utah School of 
      Medicine, Salt Lake City, UT 84132, USA.
FAU - Salama, Mohamed E
AU  - Salama ME
AD  - Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake 
      City, UT 84108, USA; Department of Pathology, University of Utah School of 
      Medicine, Salt Lake City, UT 84132, USA.
LA  - eng
PT  - Journal Article
DEP - 20160504
PL  - United States
TA  - J Pathol Inform
JT  - Journal of pathology informatics
JID - 101528849
PMC - PMC4872483
OTO - NOTNLM
OT  - Break apart probe
OT  - GenASIs analysis system
OT  - fusion probe
EDAT- 2016/05/25 06:00
MHDA- 2016/05/25 06:01
PMCR- 2016/01/01
CRDT- 2016/05/25 06:00
PHST- 2015/11/13 00:00 [received]
PHST- 2016/03/15 00:00 [accepted]
PHST- 2016/05/25 06:00 [entrez]
PHST- 2016/05/25 06:00 [pubmed]
PHST- 2016/05/25 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - S2153-3539(22)00538-7 [pii]
AID - JPI-7-20 [pii]
AID - 10.4103/2153-3539.181764 [doi]
PST - epublish
SO  - J Pathol Inform. 2016 May 4;7:20. doi: 10.4103/2153-3539.181764. eCollection 
      2016.